biostudies-literatureHumphries LANICHD NIH HHSNIAID NIH HHSNCI NIH HHSe45276https://www.ebi.ac.uk/biostudies/studies/S-EPMC3445470biostudies-literatureUnknown7(9)<h4>Background</h4>The polarized reorganization of the T cell membrane and intracellular signaling molecules in response to T cell receptor (TCR) engagement has been implicated in the modulation of T cell development and effector responses. In siRNA-based studies Dlg1, a MAGUK scaffold protein and member of the Scribble polarity complex, has been shown to play a role in T cell polarity and TCR signal specificity, however the role of Dlg1 in T cell development and function in vivo remains unclear.<h4>Methodology/principal findings</h4>Here we present the combined data from three independently-derived dlg1-knockout mouse models; two germline deficient knockouts and one conditional knockout. While defects were not observed in T cell development, TCR-induced early phospho-signaling, actin-mediPloS oneCharacterization of in vivo Dlg1 deletion on T cell development and function.PMC34454702-T32-AI-07323P01 CA093615R01-AI067253-10R01 AI067253T32-HD07516T32 AI007323T32 HD007516Round JLRussell SMSacirbegovic FSilva OTomassian TMiceli MCMcMahon KABurkhardt JKTakamiya KHumphries LAHumbert POShaffer MHHuganir RLfalseCharacterization of in vivo Dlg1 deletion on T cell development and function.<h4>Background</h4>The polarized reorganization of the T cell membrane and intracellular signaling molecules in response to T cell receptor (TCR) engagement has been implicated in the modulation of T cell development and effector responses. In siRNA-based studies Dlg1, a MAGUK scaffold protein and member of the Scribble polarity complex, has been shown to play a role in T cell polarity and TCR signal specificity, however the role of Dlg1 in T cell development and function in vivo remains unclear.<h4>Methodology/principal findings</h4>Here we present the combined data from three independently-derived dlg1-knockout mouse models; two germline deficient knockouts and one conditional knockout. While defects were not observed in T cell development, TCR-induced early phospho-signaling, actin-medi2012-01-01T00:00:00Z20122026-04-20T03:17:20.733Z2026-04-20T03:10:17.379ZS-EPMC34454702302890210.1371/journal.pone.0045276