{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["7(9)"],"submitter":["Zhang W"],"pubmed_abstract":["Receptor tyrosine phosphatase gamma (PTPRG, or RPTPγ) is a mammalian receptor-like tyrosine phosphatase which is highly expressed in the nervous system as well as other tissues. Its function and biochemical characteristics remain largely unknown. We created a knockdown (KD) line of this gene in mouse by retroviral insertion that led to 98-99% reduction of RPTPγ gene expression. The knockdown mice displayed antidepressive-like behaviors in the tail-suspension test, confirming observations by Lamprianou et al. 2006. We investigated this phenotype in detail using multiple behavioral assays. To see if the antidepressive-like phenotype was due to the loss of phosphatase activity, we made a knock-in (KI) mouse in which a mutant, RPTPγ C1060S, replaced the wild type. We showed that human wild typ"],"journal":["PloS one"],"pagination":["e45500"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3447766"],"repository":["biostudies-literature"],"pubmed_title":["Characterization of PTPRG in knockdown and phosphatase-inactive mutant mice and substrate trapping analysis of PTPRG in mammalian cells."],"pmcid":["PMC3447766"],"pubmed_authors":["Zhang W","Pogorelov VM","Hu S","Xu N","Lanthorn TH","Tran DT","Savelieva KV","Rajan I","Baker KB","Platt KA","Cullinan EB"],"additional_accession":[]},"is_claimable":false,"name":"Characterization of PTPRG in knockdown and phosphatase-inactive mutant mice and substrate trapping analysis of PTPRG in mammalian cells.","description":"Receptor tyrosine phosphatase gamma (PTPRG, or RPTPγ) is a mammalian receptor-like tyrosine phosphatase which is highly expressed in the nervous system as well as other tissues. Its function and biochemical characteristics remain largely unknown. We created a knockdown (KD) line of this gene in mouse by retroviral insertion that led to 98-99% reduction of RPTPγ gene expression. The knockdown mice displayed antidepressive-like behaviors in the tail-suspension test, confirming observations by Lamprianou et al. 2006. We investigated this phenotype in detail using multiple behavioral assays. To see if the antidepressive-like phenotype was due to the loss of phosphatase activity, we made a knock-in (KI) mouse in which a mutant, RPTPγ C1060S, replaced the wild type. We showed that human wild typ","dates":{"release":"2012-01-01T00:00:00Z","publication":"2012","modification":"2025-05-29T22:30:12.476Z","creation":"2025-05-29T22:30:12.476Z"},"accession":"S-EPMC3447766","cross_references":{"pubmed":["23029056"],"doi":["10.1371/journal.pone.0045500"]}}