{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Lie TJ"],"funding":["NIGMS NIH HHS"],"pagination":["15473-8"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3458328"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["109(38)"],"pubmed_abstract":["Despite decades of study, electron flow and energy conservation in methanogenic Archaea are still not thoroughly understood. For methanogens without cytochromes, flavin-based electron bifurcation has been proposed as an essential energy-conserving mechanism that couples exergonic and endergonic reactions of methanogenesis. However, an alternative hypothesis posits that the energy-converting hydrogenase Eha provides a chemiosmosis-driven electron input to the endergonic reaction. In vivo evidence for both hypotheses is incomplete. By genetically eliminating all nonessential pathways of H(2) metabolism in the model methanogen Methanococcus maripaludis and using formate as an additional electron donor, we isolate electron flow for methanogenesis from flux through Eha. We find that Eha does no"],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pubmed_title":["Essential anaplerotic role for the energy-converting hydrogenase Eha in hydrogenotrophic methanogenesis."],"pmcid":["PMC3458328"],"funding_grant_id":["R24 GM074783","T32 GM07270","T32 GM007270"],"pubmed_authors":["Leigh JA","Whitman WB","Lie TJ","Lupa B","Costa KC","Korpole S"],"additional_accession":[]},"is_claimable":false,"name":"Essential anaplerotic role for the energy-converting hydrogenase Eha in hydrogenotrophic methanogenesis.","description":"Despite decades of study, electron flow and energy conservation in methanogenic Archaea are still not thoroughly understood. For methanogens without cytochromes, flavin-based electron bifurcation has been proposed as an essential energy-conserving mechanism that couples exergonic and endergonic reactions of methanogenesis. However, an alternative hypothesis posits that the energy-converting hydrogenase Eha provides a chemiosmosis-driven electron input to the endergonic reaction. In vivo evidence for both hypotheses is incomplete. By genetically eliminating all nonessential pathways of H(2) metabolism in the model methanogen Methanococcus maripaludis and using formate as an additional electron donor, we isolate electron flow for methanogenesis from flux through Eha. We find that Eha does no","dates":{"release":"2012-01-01T00:00:00Z","publication":"2012 Sep","modification":"2025-04-04T12:05:03.745Z","creation":"2019-03-27T00:58:23Z"},"accession":"S-EPMC3458328","cross_references":{"pubmed":["22872868"],"doi":["10.1073/pnas.1208779109"]}}