{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["65(10)"],"submitter":["Malapelle U"],"pubmed_abstract":["<h4>Background</h4>Sanger sequencing (SS) of PCR products is still the most frequent method to test colorectal cancer for KRAS mutations in routine practice.<h4>Methods</h4>An audit of SS on 1720 routine cases was carried out, taking into account age, gender, specimen type (resection vs biopsies), tumour site (primary vs metastasis), tumour stage, neoplastic cells abundance (>30% vs <30%) and fixation type (buffered formalin vs simple formalin). In a subset of 50 wild-type (WT) patients correlations between SS findings and response rate (RR), progression-free survival (PFS) and overall survival (OS) were also evaluated.<h4>Results</h4>The tests were informative in 1691 cases (98.3%). Mutations were detected in 671 cases (39.6%). No significant differences in mutation rates were observed wi"],"journal":["Journal of clinical pathology"],"pagination":["940-4"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3461636"],"repository":["biostudies-literature"],"pubmed_title":["Sanger sequencing in routine KRAS testing: a review of 1720 cases from a pathologist's perspective."],"pmcid":["PMC3461636"],"pubmed_authors":["de Luca C","Sparano L","Troncone G","Carlomagno C","Bellevicine C","Maiello FM","Riccio P","Nappi O","Salatiello M","Rispo E","Vita G","Malapelle U","De Stefano A"],"additional_accession":[]},"is_claimable":false,"name":"Sanger sequencing in routine KRAS testing: a review of 1720 cases from a pathologist's perspective.","description":"<h4>Background</h4>Sanger sequencing (SS) of PCR products is still the most frequent method to test colorectal cancer for KRAS mutations in routine practice.<h4>Methods</h4>An audit of SS on 1720 routine cases was carried out, taking into account age, gender, specimen type (resection vs biopsies), tumour site (primary vs metastasis), tumour stage, neoplastic cells abundance (>30% vs <30%) and fixation type (buffered formalin vs simple formalin). In a subset of 50 wild-type (WT) patients correlations between SS findings and response rate (RR), progression-free survival (PFS) and overall survival (OS) were also evaluated.<h4>Results</h4>The tests were informative in 1691 cases (98.3%). Mutations were detected in 671 cases (39.6%). No significant differences in mutation rates were observed wi","dates":{"release":"2012-01-01T00:00:00Z","publication":"2012 Oct","modification":"2025-04-18T11:34:44.151Z","creation":"2019-03-27T00:58:33Z"},"accession":"S-EPMC3461636","cross_references":{"pubmed":["22872705"],"doi":["10.1136/jclinpath-2012-200773"]}}