biostudies-literatureUnknown65(10)Malapelle U<h4>Background</h4>Sanger sequencing (SS) of PCR products is still the most frequent method to test colorectal cancer for KRAS mutations in routine practice.<h4>Methods</h4>An audit of SS on 1720 routine cases was carried out, taking into account age, gender, specimen type (resection vs biopsies), tumour site (primary vs metastasis), tumour stage, neoplastic cells abundance (>30% vs <30%) and fixation type (buffered formalin vs simple formalin). In a subset of 50 wild-type (WT) patients correlations between SS findings and response rate (RR), progression-free survival (PFS) and overall survival (OS) were also evaluated.<h4>Results</h4>The tests were informative in 1691 cases (98.3%). Mutations were detected in 671 cases (39.6%). No significant differences in mutation rates were observed wiJournal of clinical pathology940-4https://www.ebi.ac.uk/biostudies/studies/S-EPMC3461636biostudies-literatureSanger sequencing in routine KRAS testing: a review of 1720 cases from a pathologist's perspective.PMC3461636de Luca CSparano LTroncone GCarlomagno CBellevicine CMaiello FMRiccio PNappi OSalatiello MRispo EVita GMalapelle UDe Stefano AfalseSanger sequencing in routine KRAS testing: a review of 1720 cases from a pathologist's perspective.<h4>Background</h4>Sanger sequencing (SS) of PCR products is still the most frequent method to test colorectal cancer for KRAS mutations in routine practice.<h4>Methods</h4>An audit of SS on 1720 routine cases was carried out, taking into account age, gender, specimen type (resection vs biopsies), tumour site (primary vs metastasis), tumour stage, neoplastic cells abundance (>30% vs <30%) and fixation type (buffered formalin vs simple formalin). In a subset of 50 wild-type (WT) patients correlations between SS findings and response rate (RR), progression-free survival (PFS) and overall survival (OS) were also evaluated.<h4>Results</h4>The tests were informative in 1691 cases (98.3%). Mutations were detected in 671 cases (39.6%). No significant differences in mutation rates were observed wi2012-01-01T00:00:00Z2012 Oct2025-04-18T11:34:44.151Z2019-03-27T00:58:33ZS-EPMC34616362287270510.1136/jclinpath-2012-200773