biostudies-literatureUnknown13(11)Roberti MPTriple negative breast cancers (TNBC) lacking hormone receptors and HER-2 amplification are very aggressive tumors. Since relevant differences between primary tumors and metastases could arise during tumor progression as evidenced by phenotypic discordances reported for hormonal receptors or HER-2 expression, in this analysis we studied changes that occurred in our TNBC model IIB-BR-G throughout the development of IIB-BR-G-MTS6 metastasis to the lymph nodes (LN) in nude mice, using an antibody-based protein array to characterize their expression profile. We also analyzed their growth kinetics, migration, invasiveness and cytoskeleton structure in vitro and in vivo. In vitro IIB-BR-G-MTS6 cells grew slower but showed higher anchorage independent growth. In vivo IIB-BR-G-MTS6 tumors grew sCancer biology & therapy1123-40https://www.ebi.ac.uk/biostudies/studies/S-EPMC3461818biostudies-literatureProtein expression changes during human triple negative breast cancer cell line progression to lymph node metastasis in a xenografted model in nude mice.PMC3461818Arriaga JMBravo AILevy EMQuinta HRBianchini MRoberti MPBarrio MMMordoh JfalseProtein expression changes during human triple negative breast cancer cell line progression to lymph node metastasis in a xenografted model in nude mice.Triple negative breast cancers (TNBC) lacking hormone receptors and HER-2 amplification are very aggressive tumors. Since relevant differences between primary tumors and metastases could arise during tumor progression as evidenced by phenotypic discordances reported for hormonal receptors or HER-2 expression, in this analysis we studied changes that occurred in our TNBC model IIB-BR-G throughout the development of IIB-BR-G-MTS6 metastasis to the lymph nodes (LN) in nude mice, using an antibody-based protein array to characterize their expression profile. We also analyzed their growth kinetics, migration, invasiveness and cytoskeleton structure in vitro and in vivo. In vitro IIB-BR-G-MTS6 cells grew slower but showed higher anchorage independent growth. In vivo IIB-BR-G-MTS6 tumors grew s2012-01-01T00:00:00Z2012 Sep2025-07-03T03:04:46.075Z2025-07-03T03:04:46.075ZS-EPMC34618182282532610.4161/cbt.21187