{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["32(6)"],"submitter":["Braeuning A"],"pubmed_abstract":["Photinus pyralis (firefly) luciferase is widely used as a reporter system to monitor alterations in gene promoter and/or signalling pathway activities in vitro. The enzyme catalyses the formation of oxyluciferin from D-luciferin in an ATP-consuming reaction involving photon emission. The purpose of the present study was to characterize the luciferase-inhibiting potential of (E)-2-fluoro-4'-methoxystilbene, which is known as a potent inhibitor of the NF-κB (nuclear factor κB) signalling pathway that is used to modulate the NF-κB signalling pathway in vitro. Results show that (E)-2-fluoro-4'-methoxystilbene effectively inhibits firefly luciferase activity in cell lysates and living cells in a non-competitive manner with respect to the luciferase substrates D-luciferin and ATP. By contrast, t"],"journal":["Bioscience reports"],"pagination":["531-7"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3475454"],"repository":["biostudies-literature"],"pubmed_title":["The nuclear factor κB inhibitor (E)-2-fluoro-4'-methoxystilbene inhibits firefly luciferase."],"pmcid":["PMC3475454"],"pubmed_authors":["Vetter S","Braeuning A"],"additional_accession":[]},"is_claimable":false,"name":"The nuclear factor κB inhibitor (E)-2-fluoro-4'-methoxystilbene inhibits firefly luciferase.","description":"Photinus pyralis (firefly) luciferase is widely used as a reporter system to monitor alterations in gene promoter and/or signalling pathway activities in vitro. The enzyme catalyses the formation of oxyluciferin from D-luciferin in an ATP-consuming reaction involving photon emission. The purpose of the present study was to characterize the luciferase-inhibiting potential of (E)-2-fluoro-4'-methoxystilbene, which is known as a potent inhibitor of the NF-κB (nuclear factor κB) signalling pathway that is used to modulate the NF-κB signalling pathway in vitro. Results show that (E)-2-fluoro-4'-methoxystilbene effectively inhibits firefly luciferase activity in cell lysates and living cells in a non-competitive manner with respect to the luciferase substrates D-luciferin and ATP. By contrast, t","dates":{"release":"2012-01-01T00:00:00Z","publication":"2012 Dec","modification":"2025-04-18T11:54:48.293Z","creation":"2019-03-27T00:59:14Z"},"accession":"S-EPMC3475454","cross_references":{"pubmed":["22789175"],"doi":["10.1042/BSR20120043"]}}