biostudies-literatureUnknown12Wu X<h4>Background</h4>Microbial lipases particularly Pseudomonas lipases are widely used for biotechnological applications. It is a meaningful work to design experiments to obtain high-level active lipase. There is a limiting factor for functional overexpression of the Pseudomonas lipase that a chaperone is necessary for effective folding. As previously reported, several methods had been used to resolve the problem. In this work, the lipase (LipA) and its chaperone (LipB) from a screened strain named AB which belongs to Pseudomonas aeruginosa were overexpressed in E. coli with two dual expression plasmid systems to enhance the production of the active lipase LipA without in vitro refolding process.<h4>Results</h4>In this work, we screened a lipase-produced strain named AB through the screeninBMC biotechnology58https://www.ebi.ac.uk/biostudies/studies/S-EPMC3497882biostudies-literatureIn vivo functional expression of a screened P. aeruginosa chaperone-dependent lipase in E. coli.PMC3497882Su EGao BYou PWei DXu JWu XfalseIn vivo functional expression of a screened P. aeruginosa chaperone-dependent lipase in E. coli.<h4>Background</h4>Microbial lipases particularly Pseudomonas lipases are widely used for biotechnological applications. It is a meaningful work to design experiments to obtain high-level active lipase. There is a limiting factor for functional overexpression of the Pseudomonas lipase that a chaperone is necessary for effective folding. As previously reported, several methods had been used to resolve the problem. In this work, the lipase (LipA) and its chaperone (LipB) from a screened strain named AB which belongs to Pseudomonas aeruginosa were overexpressed in E. coli with two dual expression plasmid systems to enhance the production of the active lipase LipA without in vitro refolding process.<h4>Results</h4>In this work, we screened a lipase-produced strain named AB through the screenin2012-01-01T00:00:00Z2012 Sep2026-05-02T19:31:15.603Z2019-03-27T01:00:32ZS-EPMC34978822295059910.1186/1472-6750-12-58