{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["32(24)"],"submitter":["Manfrini N"],"pubmed_abstract":["The Mec1 and Rad53 protein kinases are essential for budding yeast cell viability and are also required to activate the S-phase checkpoint, which supports DNA replication under stress conditions. Whether these two functions are related to each other remains to be determined, and the nature of the replication stress-dependent lethality of mec1 and rad53 mutants is still unclear. We show here that a decrease in cyclin-dependent kinase 1 (Cdk1) activity alleviates the lethal effects of mec1 and rad53 mutations both in the absence and in the presence of replication stress, indicating that the execution of a certain Cdk1-mediated event(s) is detrimental in the absence of Mec1 and Rad53. This lethality involves Cdk1 functions in both G(1) and mitosis. In fact, delaying either the G(1)/S transiti"],"journal":["Molecular and cellular biology"],"pagination":["4971-85"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3510536"],"repository":["biostudies-literature"],"pubmed_title":["G(1)/S and G(2)/M cyclin-dependent kinase activities commit cells to death in the absence of the S-phase checkpoint."],"pmcid":["PMC3510536"],"pubmed_authors":["Trovesi C","Gobbini E","Lucchini G","Manfrini N","Longhese MP","Baldo V"],"additional_accession":[]},"is_claimable":false,"name":"G(1)/S and G(2)/M cyclin-dependent kinase activities commit cells to death in the absence of the S-phase checkpoint.","description":"The Mec1 and Rad53 protein kinases are essential for budding yeast cell viability and are also required to activate the S-phase checkpoint, which supports DNA replication under stress conditions. Whether these two functions are related to each other remains to be determined, and the nature of the replication stress-dependent lethality of mec1 and rad53 mutants is still unclear. We show here that a decrease in cyclin-dependent kinase 1 (Cdk1) activity alleviates the lethal effects of mec1 and rad53 mutations both in the absence and in the presence of replication stress, indicating that the execution of a certain Cdk1-mediated event(s) is detrimental in the absence of Mec1 and Rad53. This lethality involves Cdk1 functions in both G(1) and mitosis. In fact, delaying either the G(1)/S transiti","dates":{"release":"2012-01-01T00:00:00Z","publication":"2012 Dec","modification":"2025-05-29T22:28:38.075Z","creation":"2025-05-29T22:28:38.075Z"},"accession":"S-EPMC3510536","cross_references":{"pubmed":["23045388"],"doi":["10.1128/mcb.00956-12","10.1128/MCB.00956-12"]}}