biostudies-literatureBacchetta JNCATS NIH HHSNIDDK NIH HHSNCRR NIH HHSNIAID NIH HHSNIAMS NIH HHS46-55https://www.ebi.ac.uk/biostudies/studies/S-EPMC3511915biostudies-literatureUnknown28(1)Vitamin D is a potent stimulator of monocyte innate immunity, and this effect is mediated via intracrine conversion of 25-hydroxyvitamin D (25OHD) to 1,25-dihydroxyvitamin D (1,25(OH)(2) D). In the kidney, synthesis of 1,25(OH)(2) D is suppressed by fibroblast growth factor 23 (FGF23), via transcriptional suppression of the vitamin D-activating enzyme 1α-hydroxylase (CYP27B1). We hypothesized that FGF23 also suppresses CYP27B1 in monocytes, with concomitant effects on intracrine responses to 1,25(OH)(2) D. Healthy donor peripheral blood mononuclear cell monocytes (PBMCm) and peritoneal dialysate monocyte (PDm) effluent from kidney disease patients were assessed at baseline to confirm the presence of mRNA for FGF23 receptors (FGFRs), with Klotho and FGFR1 being more strongly expressed than Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral ResearchFibroblast growth factor 23 inhibits extrarenal synthesis of 1,25-dihydroxyvitamin D in human monocytes.PMC3511915P50 AR063020P30 AI028697K23 DK080984UL1 TR000124R01 DK067563DK 67563UL1 RR033176R21 DK091672DK 80984DK 35423R01 DK035423DK0911672Bacchetta JLisse TSGales BHewison MWesseling-Perry KSalusky IBAdams JSChun RFSea JLfalseFibroblast growth factor 23 inhibits extrarenal synthesis of 1,25-dihydroxyvitamin D in human monocytes.Vitamin D is a potent stimulator of monocyte innate immunity, and this effect is mediated via intracrine conversion of 25-hydroxyvitamin D (25OHD) to 1,25-dihydroxyvitamin D (1,25(OH)(2) D). In the kidney, synthesis of 1,25(OH)(2) D is suppressed by fibroblast growth factor 23 (FGF23), via transcriptional suppression of the vitamin D-activating enzyme 1α-hydroxylase (CYP27B1). We hypothesized that FGF23 also suppresses CYP27B1 in monocytes, with concomitant effects on intracrine responses to 1,25(OH)(2) D. Healthy donor peripheral blood mononuclear cell monocytes (PBMCm) and peritoneal dialysate monocyte (PDm) effluent from kidney disease patients were assessed at baseline to confirm the presence of mRNA for FGF23 receptors (FGFRs), with Klotho and FGFR1 being more strongly expressed than 2013-01-01T00:00:00Z2013 Jan2025-04-03T23:34:06.932Z2019-03-27T01:01:18ZS-EPMC35119152288672010.1002/jbmr.1740