biostudies-literatureSchneider KSwiss National Science Foundation1138-44https://www.ebi.ac.uk/biostudies/studies/S-EPMC3512403biostudies-literatureUnknown13(12)In mammals, transcriptional autorepression by Period (PER) and Cryptochrome (CRY) protein complexes is essential for the generation of circadian rhythms. We have identified CAVIN-3 as a new, cytoplasmic PER2-interacting protein influencing circadian clock properties. Thus, CAVIN-3 loss- and gain-of-function shortened and lengthened, respectively, the circadian period in fibroblasts and affected PER:CRY protein abundance and interaction. While depletion of protein kinase Cδ (PKCδ), a known partner of CAVIN-3, had little effect on circadian gene expression, CAVIN-3 required the PKCδ-binding site to exert its effect on period length. This suggests the involvement of yet uncharacterized protein kinases. Finally, CAVIN-3 activity in circadian gene expression was independent of caveolae.EMBO reportsCAVIN-3 regulates circadian period length and PER:CRY protein abundance and interactions.PMC3512403128399113565128656Kurzchalia TSchibler UGatfield DSchneider KKocher TAndersin TfalseCAVIN-3 regulates circadian period length and PER:CRY protein abundance and interactions.In mammals, transcriptional autorepression by Period (PER) and Cryptochrome (CRY) protein complexes is essential for the generation of circadian rhythms. We have identified CAVIN-3 as a new, cytoplasmic PER2-interacting protein influencing circadian clock properties. Thus, CAVIN-3 loss- and gain-of-function shortened and lengthened, respectively, the circadian period in fibroblasts and affected PER:CRY protein abundance and interaction. While depletion of protein kinase Cδ (PKCδ), a known partner of CAVIN-3, had little effect on circadian gene expression, CAVIN-3 required the PKCδ-binding site to exert its effect on period length. This suggests the involvement of yet uncharacterized protein kinases. Finally, CAVIN-3 activity in circadian gene expression was independent of caveolae.2012-01-01T00:00:00Z2012 Dec2025-04-19T12:22:24.339Z2019-03-27T01:01:20ZS-EPMC35124032307972710.1038/embor.2012.158