biostudies-literatureBarua DNIGMS NIH HHSe51669https://www.ebi.ac.uk/biostudies/studies/S-EPMC3524258biostudies-literatureUnknown7(12)We present a model of the early events in mast cell signaling mediated by FcεRI where the plasma membrane is composed of many small ordered lipid domains (rafts), surrounded by a non-order region of lipids consisting of the remaining plasma membrane. The model treats the rafts as transient structures that constantly form and breakup, but that maintain a fixed average number per cell. The rafts have a high propensity for harboring Lyn kinase, aggregated, but not unaggregated receptors, and the linker for the activation of T cells (LAT). Phosphatase activity in the rafts is substantially reduced compared to the nonraft region. We use the model to analyze published experiments on the rat basophilic leukemia (RBL)-2H3 cell line that seem to contradict the notion that rafts offer protection. InPloS oneA mechanistic model of early FcεRI signaling: lipid rafts and the question of protection from dephosphorylation.PMC3524258R37-GM035556R37 GM035556Goldstein BBarua DfalseA mechanistic model of early FcεRI signaling: lipid rafts and the question of protection from dephosphorylation.We present a model of the early events in mast cell signaling mediated by FcεRI where the plasma membrane is composed of many small ordered lipid domains (rafts), surrounded by a non-order region of lipids consisting of the remaining plasma membrane. The model treats the rafts as transient structures that constantly form and breakup, but that maintain a fixed average number per cell. The rafts have a high propensity for harboring Lyn kinase, aggregated, but not unaggregated receptors, and the linker for the activation of T cells (LAT). Phosphatase activity in the rafts is substantially reduced compared to the nonraft region. We use the model to analyze published experiments on the rat basophilic leukemia (RBL)-2H3 cell line that seem to contradict the notion that rafts offer protection. In2012-01-01T00:00:00Z20122025-05-29T22:28:56.447Z2025-05-29T22:28:56.447ZS-EPMC35242582328473510.1371/journal.pone.0051669