{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Salimi H"],"funding":["U.S. National Institutes of Health/NIAID","Iranian Ministry of Health and Medical Education","NIAID NIH HHS","Sir Zelman Cowen Fellowship Fund, Burnet Institute","NHMRC","Sir Zelman Cowen Senior Research Fellow","Australian NHMRC Early Career Research Fellowship","Australian NHMRC Level 2 Biomedical Career Development Award"],"pagination":["113-26"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3525831"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["93(1)"],"pubmed_abstract":["BR-derived HIV-1 strains have an exceptional ability to enter macrophages via mechanisms involving their gp120 Env that remain incompletely understood. Here, we used cell-based affinity-profiling methods and mathematical modeling to generate quantitative VERSA metrics that simultaneously measure Env-CD4 and Env-CCR5 interactions. These metrics were analyzed to distinguish the phenotypes of M-tropic and non-M-tropic CCR5-using HIV-1 variants derived from autopsy BRs and LNs, respectively. We show that highly M-tropic Env variants derived from brain can be defined by two distinct and simultaneously occurring phenotypes. First, BR-derived Envs demonstrated an enhanced ability to interact with CD4 compared with LN-derived Envs, permitting entry into cells expressing scant levels of CD4. Second"],"journal":["Journal of leukocyte biology"],"pubmed_title":["Macrophage-tropic HIV-1 variants from brain demonstrate alterations in the way gp120 engages both CD4 and CCR5."],"pmcid":["PMC3525831"],"funding_grant_id":["R21 AI092218","#603708","#1006534"],"pubmed_authors":["Chikere K","Lee B","Gray LR","Ellett A","Ramsland PA","Churchill MJ","Sterjovski J","Wesselingh SL","Salimi H","Roche M","Gorry PR","Webb N"],"additional_accession":[]},"is_claimable":false,"name":"Macrophage-tropic HIV-1 variants from brain demonstrate alterations in the way gp120 engages both CD4 and CCR5.","description":"BR-derived HIV-1 strains have an exceptional ability to enter macrophages via mechanisms involving their gp120 Env that remain incompletely understood. Here, we used cell-based affinity-profiling methods and mathematical modeling to generate quantitative VERSA metrics that simultaneously measure Env-CD4 and Env-CCR5 interactions. These metrics were analyzed to distinguish the phenotypes of M-tropic and non-M-tropic CCR5-using HIV-1 variants derived from autopsy BRs and LNs, respectively. We show that highly M-tropic Env variants derived from brain can be defined by two distinct and simultaneously occurring phenotypes. First, BR-derived Envs demonstrated an enhanced ability to interact with CD4 compared with LN-derived Envs, permitting entry into cells expressing scant levels of CD4. Second","dates":{"release":"2013-01-01T00:00:00Z","publication":"2013 Jan","modification":"2026-05-05T16:59:23.688Z","creation":"2026-04-07T21:52:04.411Z"},"accession":"S-EPMC3525831","cross_references":{"pubmed":["23077246"],"doi":["10.1189/jlb.0612308"]}}