{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["7(12)"],"submitter":["Patnaik BB"],"pubmed_abstract":["<h4>Background</h4>Silkworm fecal matter is considered one of the richest sources of antimicrobial and antiviral protein (substances) and such economically feasible and eco-friendly proteins acting as secondary metabolites from the insect system can be explored for their practical utility in conferring broad spectrum disease resistance against pathogenic microbial specimens.<h4>Methodology/principal findings</h4>Silkworm fecal matter extracts prepared in 0.02 M phosphate buffer saline (pH 7.4), at a temperature of 60°C was subjected to 40% saturated ammonium sulphate precipitation and purified by gel-filtration chromatography (GFC). SDS-PAGE under denaturing conditions showed a single band at about 21.5 kDa. The peak fraction, thus obtained by GFC wastested for homogeneityusing C18reverse-"],"journal":["PloS one"],"pagination":["e50900"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3526618"],"repository":["biostudies-literature"],"pubmed_title":["Molecular cloning and characterization of novel Morus alba germin-like protein gene which encodes for a silkworm gut digestion-resistant antimicrobial protein."],"pmcid":["PMC3526618"],"pubmed_authors":["Kim DH","Saha AK","Bindroo BB","Han YS","Oh SH","Song YS","Chanh ND","Patnaik BB","Jung WJ","Kim JS"],"additional_accession":[]},"is_claimable":false,"name":"Molecular cloning and characterization of novel Morus alba germin-like protein gene which encodes for a silkworm gut digestion-resistant antimicrobial protein.","description":"<h4>Background</h4>Silkworm fecal matter is considered one of the richest sources of antimicrobial and antiviral protein (substances) and such economically feasible and eco-friendly proteins acting as secondary metabolites from the insect system can be explored for their practical utility in conferring broad spectrum disease resistance against pathogenic microbial specimens.<h4>Methodology/principal findings</h4>Silkworm fecal matter extracts prepared in 0.02 M phosphate buffer saline (pH 7.4), at a temperature of 60°C was subjected to 40% saturated ammonium sulphate precipitation and purified by gel-filtration chromatography (GFC). SDS-PAGE under denaturing conditions showed a single band at about 21.5 kDa. The peak fraction, thus obtained by GFC wastested for homogeneityusing C18reverse-","dates":{"release":"2012-01-01T00:00:00Z","publication":"2012","modification":"2025-04-21T15:30:23.892Z","creation":"2019-03-26T23:19:16Z"},"accession":"S-EPMC3526618","cross_references":{"pubmed":["23284650"],"doi":["10.1371/journal.pone.0050900"]}}