{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["1"],"submitter":["Okamoto S"],"pubmed_abstract":["Adoptive immunotherapy using TCR gene-modified T-lymphocytes is an attractive strategy for targeting malignancies. However, TCR mispairings between endogenous and introduced TCR chains are a major concern, as they may induce mixed TCRs with unknown specificities and may reduce the expression of therapeutic TCRs. To overcome these problems, we have recently established a novel retroviral siTCR vector encoding small-interfering RNAs (siRNAs) to knockdown endogenous TCR genes for the efficient expression of therapeutic TCRs. In this study, to improve the efficacy of siTCR vectors, we developed 2A peptide-based siTCR vectors that could increase the expression levels of transduced TCRs compared with internal promoter-based siTCR vectors. We also evaluated the efficacy of an siTCR strategy and t"],"journal":["Molecular therapy. Nucleic acids"],"pagination":["e63"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3528300"],"repository":["biostudies-literature"],"pubmed_title":["A Promising Vector for TCR Gene Therapy: Differential Effect of siRNA, 2A Peptide, and Disulfide Bond on the Introduced TCR Expression."],"pmcid":["PMC3528300"],"pubmed_authors":["Shiku H","Ikeda H","Goto Y","Amaishi Y","Kuzushima K","Fujiwara H","Yasukawa M","Okamoto S","Mineno J"],"additional_accession":[]},"is_claimable":false,"name":"A Promising Vector for TCR Gene Therapy: Differential Effect of siRNA, 2A Peptide, and Disulfide Bond on the Introduced TCR Expression.","description":"Adoptive immunotherapy using TCR gene-modified T-lymphocytes is an attractive strategy for targeting malignancies. However, TCR mispairings between endogenous and introduced TCR chains are a major concern, as they may induce mixed TCRs with unknown specificities and may reduce the expression of therapeutic TCRs. To overcome these problems, we have recently established a novel retroviral siTCR vector encoding small-interfering RNAs (siRNAs) to knockdown endogenous TCR genes for the efficient expression of therapeutic TCRs. In this study, to improve the efficacy of siTCR vectors, we developed 2A peptide-based siTCR vectors that could increase the expression levels of transduced TCRs compared with internal promoter-based siTCR vectors. We also evaluated the efficacy of an siTCR strategy and t","dates":{"release":"2012-01-01T00:00:00Z","publication":"2012 Dec","modification":"2025-04-18T16:19:41.815Z","creation":"2019-03-27T01:02:04Z"},"accession":"S-EPMC3528300","cross_references":{"pubmed":["23250361"],"doi":["10.1038/mtna.2012.52"]}}