{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["7(12)"],"submitter":["Racke FK"],"pubmed_abstract":["Thrombopoiesis following severe bone marrow injury frequently is delayed, thereby resulting in life-threatening thrombocytopenia for which there are limited treatment options. The reasons for these delays in recovery are not well understood. Protein kinase C (PKC) agonists promote megakaryocyte differentiation in leukemia cell lines and primary cells. However, little is known about the megakaryopoietic effects of PKC agonists on primary CD34+ cells grown in culture or in vivo. Here we present evidence that the novel PKC isoform-selective agonist 3,20 ingenol dibenzoate (IDB) potently stimulates early megakaryopoiesis of human CD34+ cells. In contrast, broad spectrum PKC agonists failed to do so. In vivo, a single intraperitoneal injection of IDB selectively increased platelets in mice with"],"journal":["PloS one"],"pagination":["e51059"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3528756"],"repository":["biostudies-literature"],"pubmed_title":["Unique in vitro and in vivo thrombopoietic activities of ingenol 3,20 dibenzoate, a Ca(++)-independent protein kinase C isoform agonist."],"pmcid":["PMC3528756"],"pubmed_authors":["Yang W","Rutledge H","Baird M","Racke FK","Barth RF","Huo T","Gupta N","Weldon M"],"additional_accession":[]},"is_claimable":false,"name":"Unique in vitro and in vivo thrombopoietic activities of ingenol 3,20 dibenzoate, a Ca(++)-independent protein kinase C isoform agonist.","description":"Thrombopoiesis following severe bone marrow injury frequently is delayed, thereby resulting in life-threatening thrombocytopenia for which there are limited treatment options. The reasons for these delays in recovery are not well understood. Protein kinase C (PKC) agonists promote megakaryocyte differentiation in leukemia cell lines and primary cells. However, little is known about the megakaryopoietic effects of PKC agonists on primary CD34+ cells grown in culture or in vivo. Here we present evidence that the novel PKC isoform-selective agonist 3,20 ingenol dibenzoate (IDB) potently stimulates early megakaryopoiesis of human CD34+ cells. In contrast, broad spectrum PKC agonists failed to do so. In vivo, a single intraperitoneal injection of IDB selectively increased platelets in mice with","dates":{"release":"2012-01-01T00:00:00Z","publication":"2012","modification":"2025-05-31T22:54:17.491Z","creation":"2025-05-31T22:54:17.491Z"},"accession":"S-EPMC3528756","cross_references":{"pubmed":["23284657"],"doi":["10.1371/journal.pone.0051059"]}}