{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["8(1)"],"submitter":["Martinez-Palacian A"],"pubmed_abstract":["We have previously shown that oval cells harboring a genetically inactivated Met tyrosine kinase (Met(-/-) oval cells) are more sensitive to TGF-β-induced apoptosis than cells expressing a functional Met (Met(flx/flx)), demonstrating that the HGF/Met axis plays a pivotal role in oval cell survival. Here, we have examined the mechanism behind this effect and have found that TGF-β induced a mitochondria-dependent apoptotic cell death in Met(flx/flx) and Met(-/-) oval cells, associated with a marked increase in levels of the BH3-only proteins Bim and Bmf. Bmf plays a key role during TGF-β-mediated apoptosis since knocking down of BMF significantly diminished the apoptotic response in Met(-/-) oval cells. TGF-β also induced oxidative stress accompanied by NADPH oxidase 4 (Nox4) mRNA up-regulat"],"journal":["PloS one"],"pagination":["e53108"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3534654"],"repository":["biostudies-literature"],"pubmed_title":["Mouse hepatic oval cells require Met-dependent PI3K to impair TGF-β-induced oxidative stress and apoptosis."],"pmcid":["PMC3534654"],"pubmed_authors":["Herrera B","Fernandez M","de Morena-Frutos D","Sanchez A","Suarez-Causado A","Garcia-Alvaro M","Fabregat I","Martinez-Palacian A","Roncero C","del Castillo G"],"additional_accession":[]},"is_claimable":false,"name":"Mouse hepatic oval cells require Met-dependent PI3K to impair TGF-β-induced oxidative stress and apoptosis.","description":"We have previously shown that oval cells harboring a genetically inactivated Met tyrosine kinase (Met(-/-) oval cells) are more sensitive to TGF-β-induced apoptosis than cells expressing a functional Met (Met(flx/flx)), demonstrating that the HGF/Met axis plays a pivotal role in oval cell survival. Here, we have examined the mechanism behind this effect and have found that TGF-β induced a mitochondria-dependent apoptotic cell death in Met(flx/flx) and Met(-/-) oval cells, associated with a marked increase in levels of the BH3-only proteins Bim and Bmf. Bmf plays a key role during TGF-β-mediated apoptosis since knocking down of BMF significantly diminished the apoptotic response in Met(-/-) oval cells. TGF-β also induced oxidative stress accompanied by NADPH oxidase 4 (Nox4) mRNA up-regulat","dates":{"release":"2013-01-01T00:00:00Z","publication":"2013","modification":"2025-04-04T08:31:34.686Z","creation":"2019-03-26T23:19:18Z"},"accession":"S-EPMC3534654","cross_references":{"pubmed":["23301029"],"doi":["10.1371/journal.pone.0053108"]}}