biostudies-literatureUnknown87(1)Krug AKDevelopmental neurotoxicity (DNT) and many forms of reproductive toxicity (RT) often manifest themselves in functional deficits that are not necessarily based on cell death, but rather on minor changes relating to cell differentiation or communication. The fields of DNT/RT would greatly benefit from in vitro tests that allow the identification of toxicant-induced changes of the cellular proteostasis, or of its underlying transcriptome network. Therefore, the 'human embryonic stem cell (hESC)-derived novel alternative test systems (ESNATS)' European commission research project established RT tests based on defined differentiation protocols of hESC and their progeny. Valproic acid (VPA) and methylmercury (MeHg) were used as positive control compounds to address the following fundamental quesArchives of toxicology123-43https://www.ebi.ac.uk/biostudies/studies/S-EPMC3535399biostudies-literatureHuman embryonic stem cell-derived test systems for developmental neurotoxicity: a transcriptomics approach.PMC3535399Jagtap SSachinidis AMarchan RKrug AKStoppini LHengstler JGRempel EKadereit SWesterhout JRahnenfuhrer JLeist MSotiriadou INatarajan KJulien SKortenkamp AVerwei MEnsenat-Waser RBosgra SEvans RMGerhard DBalmer NVHothorn Lvan Thriel CKolde RBremer SKrause KHMeganathan KZagoura DGaspar JAPeterson HVojnits KBaquie MWaldmann TWinkler JHenry MHescheler JHeke MVilo JfalseHuman embryonic stem cell-derived test systems for developmental neurotoxicity: a transcriptomics approach.Developmental neurotoxicity (DNT) and many forms of reproductive toxicity (RT) often manifest themselves in functional deficits that are not necessarily based on cell death, but rather on minor changes relating to cell differentiation or communication. The fields of DNT/RT would greatly benefit from in vitro tests that allow the identification of toxicant-induced changes of the cellular proteostasis, or of its underlying transcriptome network. Therefore, the 'human embryonic stem cell (hESC)-derived novel alternative test systems (ESNATS)' European commission research project established RT tests based on defined differentiation protocols of hESC and their progeny. Valproic acid (VPA) and methylmercury (MeHg) were used as positive control compounds to address the following fundamental ques2013-01-01T00:00:00Z2013 Jan2026-06-06T18:19:25.484Z2026-06-05T03:07:34.517ZS-EPMC35353992317975310.1007/s00204-012-0967-3