biostudies-literatureHickey CJEuropean Hematology AssociationNCI NIH HHS159-69https://www.ebi.ac.uk/biostudies/studies/S-EPMC3538328biostudies-literatureUnknown121(1)Recently, we showed that increased miR-181a expression was associated with improved outcomes in cytogenetically normal acute myeloid leukemia (CN-AML). Interestingly, miR-181a expression was increased in CN-AML patients harboring CEBPA mutations, which are usually biallelic and associate with better prognosis. CEBPA encodes the C/EBPα transcription factor. We demonstrate here that the presence of N-terminal CEBPA mutations and miR-181a expression are linked. Indeed, the truncated C/EBPα-p30 isoform, which is produced from the N-terminal mutant CEBPA gene or from the differential translation of wild-type CEBPA mRNA and is commonly believed to have no transactivation activity, binds to the miR-181a-1 promoter and up-regulates the microRNA expression. Furthermore, we show that lenalidomide, aBloodLenalidomide-mediated enhanced translation of C/EBPα-p30 protein up-regulates expression of the antileukemic microRNA-181a in acute myeloid leukemia.PMC3538328CA35279CA101140U10 CA035279U24 CA114725CA114725CA16058CA33601U10 CA003927CA31946U10 CA031946P30 CA016058U10 CA077658U10 CA101140TRTH68CA03927CA41287CA77658R01 CA095512U10 CA033601U10 CA041287P50 CA140158Vij RWhitman SPBecker HSanthanam RZhao XMrozek KNicolet DPerrotti DRadomska HSWalker ABloomfield CDFehniger TAByrd JCLee LJMarcucci GAlachkar HEiring AMMaharry KWu LCHickey CJSchwind SGarzon RBlum WMishra ADorrance AMWu YZCaligiuri MAfalseLenalidomide-mediated enhanced translation of C/EBPα-p30 protein up-regulates expression of the antileukemic microRNA-181a in acute myeloid leukemia.Recently, we showed that increased miR-181a expression was associated with improved outcomes in cytogenetically normal acute myeloid leukemia (CN-AML). Interestingly, miR-181a expression was increased in CN-AML patients harboring CEBPA mutations, which are usually biallelic and associate with better prognosis. CEBPA encodes the C/EBPα transcription factor. We demonstrate here that the presence of N-terminal CEBPA mutations and miR-181a expression are linked. Indeed, the truncated C/EBPα-p30 isoform, which is produced from the N-terminal mutant CEBPA gene or from the differential translation of wild-type CEBPA mRNA and is commonly believed to have no transactivation activity, binds to the miR-181a-1 promoter and up-regulates the microRNA expression. Furthermore, we show that lenalidomide, a2013-01-01T00:00:00Z2013 Jan2026-05-02T09:11:42.839Z2026-04-07T17:47:39.408ZS-EPMC35383282310031110.1182/blood-2012-05-428573