{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Blich M"],"funding":["NCI NIH HHS"],"pagination":["e56-65"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3548034"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["33(2)"],"pubmed_abstract":["Objective
Factors and mechanisms that activate macrophages in atherosclerotic plaques are incompletely understood. We examined the capacity of heparanase to activate macrophages.Methods and results
Highly purified heparanase was added to mouse peritoneal macrophages and macrophage-like J774 cells, and the levels of tumor necrosis factor-α, matrix metalloproteinase-9, interlukin-1, and monocyte chemotactic protein-1 were evaluated by ELISA. Gene expression was determined by RT-PCR. Cells collected from Toll-like receptor-2 and Toll-like receptor-4 knockout mice were evaluated similarly. Heparanase levels in the plasma of patients with acute myocardial infarction, stable angina, and healthy subjects were determined by ELISA. Immunohistochemistry was applied to detect the expr"],"journal":["Arteriosclerosis, thrombosis, and vascular biology"],"pubmed_title":["Macrophage activation by heparanase is mediated by TLR-2 and TLR-4 and associates with plaque progression."],"pmcid":["PMC3548034"],"funding_grant_id":["CA106456","R01 CA106456"],"pubmed_authors":["Nussbaum G","Sabo E","Petcherski S","Hammerman H","Shafat I","Blich M","Ilan N","Golan A","Aronson D","Avniel-Polak S","Arvatz G","Eitan A","Cohen-Kaplan V","Axelman E","Vlodavsky I","Sebbag A"],"additional_accession":[]},"is_claimable":false,"name":"Macrophage activation by heparanase is mediated by TLR-2 and TLR-4 and associates with plaque progression.","description":"Objective
Factors and mechanisms that activate macrophages in atherosclerotic plaques are incompletely understood. We examined the capacity of heparanase to activate macrophages.Methods and results
Highly purified heparanase was added to mouse peritoneal macrophages and macrophage-like J774 cells, and the levels of tumor necrosis factor-α, matrix metalloproteinase-9, interlukin-1, and monocyte chemotactic protein-1 were evaluated by ELISA. Gene expression was determined by RT-PCR. Cells collected from Toll-like receptor-2 and Toll-like receptor-4 knockout mice were evaluated similarly. Heparanase levels in the plasma of patients with acute myocardial infarction, stable angina, and healthy subjects were determined by ELISA. Immunohistochemistry was applied to detect the expr","dates":{"release":"2013-01-01T00:00:00Z","publication":"2013 Feb","modification":"2025-04-18T11:54:58.764Z","creation":"2019-03-27T01:03:28Z"},"accession":"S-EPMC3548034","cross_references":{"pubmed":["23162016"],"doi":["10.1161/ATVBAHA.112.254961","10.1161/atvbaha.112.254961"]}}