{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["9(2)"],"submitter":["Monastyrska I"],"pubmed_abstract":["Equine arteritis virus (EAV) is an enveloped, positive-strand RNA virus. Genome replication of EAV has been associated with modified intracellular membranes that are shaped into double-membrane vesicles (DMVs). We showed by immuno-electron microscopy that the DMVs induced in EAV-infected cells contain double-strand (ds)RNA molecules, presumed RNA replication intermediates, and are decorated with the autophagy marker protein microtubule-associated protein 1 light chain 3 (LC3). Replication of EAV, however, was not affected in autophagy-deficient cells lacking autophagy-related protein 7 (ATG7). Nevertheless, colocalization of DMVs and LC3 was still observed in these knockout cells, which only contain the nonlipidated form of LC3. Although autophagy is not required, depletion of LC3 markedly"],"journal":["Autophagy"],"pagination":["164-74"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3552881"],"repository":["biostudies-literature"],"pubmed_title":["An autophagy-independent role for LC3 in equine arteritis virus replication."],"pmcid":["PMC3552881"],"pubmed_authors":["Ulasli M","Reggiori F","Monastyrska I","Rottier PJ","de Haan CA","Guan JL"],"additional_accession":[]},"is_claimable":false,"name":"An autophagy-independent role for LC3 in equine arteritis virus replication.","description":"Equine arteritis virus (EAV) is an enveloped, positive-strand RNA virus. Genome replication of EAV has been associated with modified intracellular membranes that are shaped into double-membrane vesicles (DMVs). We showed by immuno-electron microscopy that the DMVs induced in EAV-infected cells contain double-strand (ds)RNA molecules, presumed RNA replication intermediates, and are decorated with the autophagy marker protein microtubule-associated protein 1 light chain 3 (LC3). Replication of EAV, however, was not affected in autophagy-deficient cells lacking autophagy-related protein 7 (ATG7). Nevertheless, colocalization of DMVs and LC3 was still observed in these knockout cells, which only contain the nonlipidated form of LC3. Although autophagy is not required, depletion of LC3 markedly","dates":{"release":"2013-01-01T00:00:00Z","publication":"2013 Feb","modification":"2025-04-04T18:53:53.661Z","creation":"2019-03-27T01:03:39Z"},"accession":"S-EPMC3552881","cross_references":{"pubmed":["23182945"],"doi":["10.4161/auto.22743"]}}