{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["337(1)"],"submitter":["Guard J"],"pubmed_abstract":["Two DNA-based methods were compared for the ability to assign serotype to 139 isolates of Salmonella enterica ssp. I. Intergenic sequence ribotyping (ISR) evaluated single nucleotide polymorphisms occurring in a 5S ribosomal gene region and flanking sequences bordering the gene dkgB. A DNA microarray hybridization method that assessed the presence and the absence of sets of genes was the second method. Serotype was assigned for 128 (92.1%) of submissions by the two DNA methods. ISR detected mixtures of serotypes within single colonies and it cost substantially less than Kauffmann-White serotyping and DNA microarray hybridization. Decreasing the cost of serotyping S. enterica while maintaining reliability may encourage routine testing and research."],"journal":["FEMS microbiology letters"],"pagination":["61-72"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3558799"],"repository":["biostudies-literature"],"pubmed_title":["Comparison of dkgB-linked intergenic sequence ribotyping to DNA microarray hybridization for assigning serotype to Salmonella enterica."],"pmcid":["PMC3558799"],"pubmed_authors":["Fedorka-Cray P","Guard J","Van Kessel J","Morales C","Hinton A","Stewart T","Jones D","Frye J","Sanchez-Ingunza R","Liljebjelke K","Jackson C","Gast R","Ingram K"],"additional_accession":[]},"is_claimable":false,"name":"Comparison of dkgB-linked intergenic sequence ribotyping to DNA microarray hybridization for assigning serotype to Salmonella enterica.","description":"Two DNA-based methods were compared for the ability to assign serotype to 139 isolates of Salmonella enterica ssp. I. Intergenic sequence ribotyping (ISR) evaluated single nucleotide polymorphisms occurring in a 5S ribosomal gene region and flanking sequences bordering the gene dkgB. A DNA microarray hybridization method that assessed the presence and the absence of sets of genes was the second method. Serotype was assigned for 128 (92.1%) of submissions by the two DNA methods. ISR detected mixtures of serotypes within single colonies and it cost substantially less than Kauffmann-White serotyping and DNA microarray hybridization. Decreasing the cost of serotyping S. enterica while maintaining reliability may encourage routine testing and research.","dates":{"release":"2012-01-01T00:00:00Z","publication":"2012 Dec","modification":"2021-02-19T20:23:40Z","creation":"2019-03-27T01:04:03Z"},"accession":"S-EPMC3558799","cross_references":{"pubmed":["22998607"],"doi":["10.1111/1574-6968.12010"]}}