biostudies-literatureEberlin LSNIBIB NIH HHSNCRR NIH HHSNINDS NIH HHSNCI NIH HHSNIH HHS1611-6https://www.ebi.ac.uk/biostudies/studies/S-EPMC3562800biostudies-literatureUnknown110(5)The main goal of brain tumor surgery is to maximize tumor resection while preserving brain function. However, existing imaging and surgical techniques do not offer the molecular information needed to delineate tumor boundaries. We have developed a system to rapidly analyze and classify brain tumors based on lipid information acquired by desorption electrospray ionization mass spectrometry (DESI-MS). In this study, a classifier was built to discriminate gliomas and meningiomas based on 36 glioma and 19 meningioma samples. The classifier was tested and results were validated for intraoperative use by analyzing and diagnosing tissue sections from 32 surgical specimens obtained from five research subjects who underwent brain tumor resection. The samples analyzed included oligodendroglioma, astProceedings of the National Academy of Sciences of the United States of AmericaAmbient mass spectrometry for the intraoperative molecular diagnosis of human brain tumors.PMC3562800K08 NS064168P41EB015898K08NS0641681DP2OD007383-01P41 EB015898R21 EB0094591R21EB009459P41 RR019703P41RR019703R25 CA089017DP2 OD007383Eberlin LSLigon KLGolby AJOrringer DDunn IFNorton IJolesz FACooks RGLiu XSantagata SJarmusch AKAgar NYIde JLfalseAmbient mass spectrometry for the intraoperative molecular diagnosis of human brain tumors.The main goal of brain tumor surgery is to maximize tumor resection while preserving brain function. However, existing imaging and surgical techniques do not offer the molecular information needed to delineate tumor boundaries. We have developed a system to rapidly analyze and classify brain tumors based on lipid information acquired by desorption electrospray ionization mass spectrometry (DESI-MS). In this study, a classifier was built to discriminate gliomas and meningiomas based on 36 glioma and 19 meningioma samples. The classifier was tested and results were validated for intraoperative use by analyzing and diagnosing tissue sections from 32 surgical specimens obtained from five research subjects who underwent brain tumor resection. The samples analyzed included oligodendroglioma, ast2013-01-01T00:00:00Z2013 Jan2026-07-16T16:32:13.003Z2026-07-09T11:10:19.598ZS-EPMC35628002330028510.1073/pnas.1215687110