{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Collins HF"],"funding":["NIGMS NIH HHS","Biotechnology and Biological Sciences Research Council"],"pagination":["e55708"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3573010"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["8(2)"],"pubmed_abstract":["Despite the extensive study of the biosynthesis of the complex molecule B12 (cobalamin), the mechanism by which the lower ligand 5,6-dimethylbenzimidazole (DMB) is formed has remained something of a mystery. However, recent work has identified and characterized a DMB-synthase (BluB) responsible for the oxygen-dependent, single enzyme conversion of FMN to DMB. In this work, we have identified BluB homologs from the aerobic purple, nonsulfur, photosynthetic bacterium Rhodobacter capsulatus and the aerobic soil bacterium Bacillus megaterium and have demonstrated DMB synthesis by the use of a novel complementation assay in which a B12 deficient strain, substituted with the precursor cobinamide is recovered either by the addition of DMB or by the recombinant expression of a bluB gene. The DMB-s"],"journal":["PloS one"],"pubmed_title":["Bacillus megaterium has both a functional BluB protein required for DMB synthesis and a related flavoprotein that forms a stable radical species."],"pmcid":["PMC3573010"],"funding_grant_id":["R37 GM040313","BB/G014361/1","BB/E002889/1"],"pubmed_authors":["Biedendieck R","Munro AW","Lawrence AD","McLean KJ","Rigby SE","Escalante-Semerena JC","Collins HF","Leech HK","Gray M","Warren MJ"],"additional_accession":[]},"is_claimable":false,"name":"Bacillus megaterium has both a functional BluB protein required for DMB synthesis and a related flavoprotein that forms a stable radical species.","description":"Despite the extensive study of the biosynthesis of the complex molecule B12 (cobalamin), the mechanism by which the lower ligand 5,6-dimethylbenzimidazole (DMB) is formed has remained something of a mystery. However, recent work has identified and characterized a DMB-synthase (BluB) responsible for the oxygen-dependent, single enzyme conversion of FMN to DMB. In this work, we have identified BluB homologs from the aerobic purple, nonsulfur, photosynthetic bacterium Rhodobacter capsulatus and the aerobic soil bacterium Bacillus megaterium and have demonstrated DMB synthesis by the use of a novel complementation assay in which a B12 deficient strain, substituted with the precursor cobinamide is recovered either by the addition of DMB or by the recombinant expression of a bluB gene. The DMB-s","dates":{"release":"2013-01-01T00:00:00Z","publication":"2013","modification":"2025-04-19T17:20:26.625Z","creation":"2019-03-26T23:19:47Z"},"accession":"S-EPMC3573010","cross_references":{"pubmed":["23457476"],"doi":["10.1371/journal.pone.0055708"]}}