biostudies-literatureKnowles LMNCATS NIH HHSNCI NIH HHS194-203https://www.ebi.ac.uk/biostudies/studies/S-EPMC3579011biostudies-literatureUnknown11(2)High-grade non-muscle-invasive bladder cancer is commonly treated with Bacillus Calmette-Guérin, an immunotherapeutic that depends on fibronectin and tumor cell integrin α5β1 for internalization into bladder cancer cells. We previously showed that the anti-angiogenic peptide CLT1 forms cytotoxic complexes with fibronectin that are cooperatively internalized into proliferating endothelium through ligation of integrins and chloride intracellular channel 1. While CLT1 has no effect on mature, differentiated cells, we show here that CLT1 is highly cytotoxic for a panel of bladder tumor cell lines as well as a variety of cell lines derived from kidney, lung, breast, and prostate cancer. Paralleling our previous results, we found CLT1-induced tumor cell death to be increased in the presence of fMolecular cancer research : MCRCLT1 targets bladder cancer through integrin α5β1 and CLIC3.PMC3579011P30 CA047904R01 CA134330CA134330P30CA047904UL1 TR000005Knowles LMGingrich JRZewe JMalik GPilch JParwani AVfalseCLT1 targets bladder cancer through integrin α5β1 and CLIC3.High-grade non-muscle-invasive bladder cancer is commonly treated with Bacillus Calmette-Guérin, an immunotherapeutic that depends on fibronectin and tumor cell integrin α5β1 for internalization into bladder cancer cells. We previously showed that the anti-angiogenic peptide CLT1 forms cytotoxic complexes with fibronectin that are cooperatively internalized into proliferating endothelium through ligation of integrins and chloride intracellular channel 1. While CLT1 has no effect on mature, differentiated cells, we show here that CLT1 is highly cytotoxic for a panel of bladder tumor cell lines as well as a variety of cell lines derived from kidney, lung, breast, and prostate cancer. Paralleling our previous results, we found CLT1-induced tumor cell death to be increased in the presence of f2013-01-01T00:00:00Z2013 Feb2026-03-27T15:33:32.818Z2025-08-24T03:08:44.235ZS-EPMC35790112320439410.1158/1541-7786.MCR-12-030010.1158/1541-7786.mcr-12-0300