{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["288(8)"],"submitter":["Pan Q"],"pubmed_abstract":["We investigated whether a synthetic tetrameric branched peptide based on the conserved TFLK motif from mammary-associated serum amyloid A3 (M-SAA3) is more efficient than the monomeric peptide at up-regulating MUC3 expression and examined the possible mechanism(s) and biological significance of this process. We used standard solid-phase methods to synthesize a tetrameric branched peptide (sequence GWLTFLKAAG) containing a trilysine core, termed the TFLK-containing 10-mer BP. The aberrant expression of transcription factors was analyzed using a transcription factor protein/DNA array. MUC3 and relevant transcription factors were detected using real-time PCR and/or Western blots. The luciferase assay, EMSA, and ChIP assays were used to analyze the activity of the human MUC3 promoter. The bact"],"journal":["The Journal of biological chemistry"],"pagination":["5407-16"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3581376"],"repository":["biostudies-literature"],"pubmed_title":["Enhanced membrane-tethered mucin 3 (MUC3) expression by a tetrameric branched peptide with a conserved TFLK motif inhibits bacteria adherence."],"pmcid":["PMC3581376"],"pubmed_authors":["Chen L","Peng Z","Song L","Li X","Liu Y","Yang Y","He Y","Wang R","Tian Y","Chen W","Mao X","Pan Q","Zhu R","Ye J"],"additional_accession":[]},"is_claimable":false,"name":"Enhanced membrane-tethered mucin 3 (MUC3) expression by a tetrameric branched peptide with a conserved TFLK motif inhibits bacteria adherence.","description":"We investigated whether a synthetic tetrameric branched peptide based on the conserved TFLK motif from mammary-associated serum amyloid A3 (M-SAA3) is more efficient than the monomeric peptide at up-regulating MUC3 expression and examined the possible mechanism(s) and biological significance of this process. We used standard solid-phase methods to synthesize a tetrameric branched peptide (sequence GWLTFLKAAG) containing a trilysine core, termed the TFLK-containing 10-mer BP. The aberrant expression of transcription factors was analyzed using a transcription factor protein/DNA array. MUC3 and relevant transcription factors were detected using real-time PCR and/or Western blots. The luciferase assay, EMSA, and ChIP assays were used to analyze the activity of the human MUC3 promoter. The bact","dates":{"release":"2013-01-01T00:00:00Z","publication":"2013 Feb","modification":"2025-04-25T21:58:53.296Z","creation":"2019-03-27T01:05:13Z"},"accession":"S-EPMC3581376","cross_references":{"pubmed":["23316049"],"doi":["10.1074/jbc.M112.408245","10.1074/jbc.m112.408245"]}}