{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wang R"],"funding":["NCI NIH HHS","NIH HHS","NIGMS NIH HHS"],"pagination":["1048-56"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3582175"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["135(3)"],"pubmed_abstract":["Protein methyltransferases (PMTs) have emerged as important epigenetic regulators in myriad biological processes in both normal physiology and disease conditions. However, elucidating PMT-regulated epigenetic processes has been hampered by ambiguous knowledge about in vivo activities of individual PMTs particularly because of their overlapping but nonredundant functions. To address limitations of conventional approaches in mapping chromatin modification of specific PMTs, we have engineered the chromatin-modifying apparatus and formulated a novel technology, termed clickable chromatin enrichment with parallel DNA sequencing (CliEn-seq), to probe genome-wide chromatin modification within living cells. The three-step approach of CliEn-seq involves in vivo synthesis of S-adenosyl-L-methionine "],"journal":["Journal of the American Chemical Society"],"pubmed_title":["Profiling genome-wide chromatin methylation with engineered posttranslation apparatus within living cells."],"pmcid":["PMC3582175"],"funding_grant_id":["DP2 OD007335","1R01GM096056","P30 CA008748","R01 GM096056","1DP2-OD007335"],"pubmed_authors":["Liu Y","Lailler N","Blum G","Wang R","Luo M","Islam K","Zheng W","Deng H","Tang H"],"additional_accession":[]},"is_claimable":false,"name":"Profiling genome-wide chromatin methylation with engineered posttranslation apparatus within living cells.","description":"Protein methyltransferases (PMTs) have emerged as important epigenetic regulators in myriad biological processes in both normal physiology and disease conditions. However, elucidating PMT-regulated epigenetic processes has been hampered by ambiguous knowledge about in vivo activities of individual PMTs particularly because of their overlapping but nonredundant functions. To address limitations of conventional approaches in mapping chromatin modification of specific PMTs, we have engineered the chromatin-modifying apparatus and formulated a novel technology, termed clickable chromatin enrichment with parallel DNA sequencing (CliEn-seq), to probe genome-wide chromatin modification within living cells. The three-step approach of CliEn-seq involves in vivo synthesis of S-adenosyl-L-methionine ","dates":{"release":"2013-01-01T00:00:00Z","publication":"2013 Jan","modification":"2026-04-29T21:47:46.085Z","creation":"2026-04-07T15:32:28.815Z"},"accession":"S-EPMC3582175","cross_references":{"pubmed":["23244065"],"doi":["10.1021/ja309412s"]}}