{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["8(2)"],"submitter":["Lu Y"],"pubmed_abstract":["A circular plasmid containing a gene coding sequence has been broadly used for studying gene regulation in cells. However, to accommodate a quick screen plasmid construction and preparation can be time consuming. Here we report a PCR amplified dsDNA fragments (PCR-fragments) based transient expression system (PCR-TES) for suiting in the study of gene regulation in plant cells. Instead of transforming plasmids into plant cells, transient expression of PCR-fragments can be applicable. The transformation efficiency and expression property of PCR-fragments are comparable to transformation using plasmids. We analyzed the transformation efficiency in PCR-TES at transcription and protein levels. Our results indicate that the PCR-TES is as versatile as the conventional transformation system using "],"journal":["PloS one"],"pagination":["e57171"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3582559"],"repository":["biostudies-literature"],"pubmed_title":["Directly transforming PCR-amplified DNA fragments into plant cells is a versatile system that facilitates the transient expression assay."],"pmcid":["PMC3582559"],"pubmed_authors":["He Y","Lu Y","Chen X","Wang Y","Wu Y"],"additional_accession":[]},"is_claimable":false,"name":"Directly transforming PCR-amplified DNA fragments into plant cells is a versatile system that facilitates the transient expression assay.","description":"A circular plasmid containing a gene coding sequence has been broadly used for studying gene regulation in cells. However, to accommodate a quick screen plasmid construction and preparation can be time consuming. Here we report a PCR amplified dsDNA fragments (PCR-fragments) based transient expression system (PCR-TES) for suiting in the study of gene regulation in plant cells. Instead of transforming plasmids into plant cells, transient expression of PCR-fragments can be applicable. The transformation efficiency and expression property of PCR-fragments are comparable to transformation using plasmids. We analyzed the transformation efficiency in PCR-TES at transcription and protein levels. Our results indicate that the PCR-TES is as versatile as the conventional transformation system using ","dates":{"release":"2013-01-01T00:00:00Z","publication":"2013","modification":"2025-04-26T10:28:00.011Z","creation":"2019-03-26T23:19:55Z"},"accession":"S-EPMC3582559","cross_references":{"pubmed":["23468926"],"doi":["10.1371/journal.pone.0057171"]}}