biostudies-literatureUnknown8(2)Lu YA circular plasmid containing a gene coding sequence has been broadly used for studying gene regulation in cells. However, to accommodate a quick screen plasmid construction and preparation can be time consuming. Here we report a PCR amplified dsDNA fragments (PCR-fragments) based transient expression system (PCR-TES) for suiting in the study of gene regulation in plant cells. Instead of transforming plasmids into plant cells, transient expression of PCR-fragments can be applicable. The transformation efficiency and expression property of PCR-fragments are comparable to transformation using plasmids. We analyzed the transformation efficiency in PCR-TES at transcription and protein levels. Our results indicate that the PCR-TES is as versatile as the conventional transformation system using PloS onee57171https://www.ebi.ac.uk/biostudies/studies/S-EPMC3582559biostudies-literatureDirectly transforming PCR-amplified DNA fragments into plant cells is a versatile system that facilitates the transient expression assay.PMC3582559He YLu YChen XWang YWu YfalseDirectly transforming PCR-amplified DNA fragments into plant cells is a versatile system that facilitates the transient expression assay.A circular plasmid containing a gene coding sequence has been broadly used for studying gene regulation in cells. However, to accommodate a quick screen plasmid construction and preparation can be time consuming. Here we report a PCR amplified dsDNA fragments (PCR-fragments) based transient expression system (PCR-TES) for suiting in the study of gene regulation in plant cells. Instead of transforming plasmids into plant cells, transient expression of PCR-fragments can be applicable. The transformation efficiency and expression property of PCR-fragments are comparable to transformation using plasmids. We analyzed the transformation efficiency in PCR-TES at transcription and protein levels. Our results indicate that the PCR-TES is as versatile as the conventional transformation system using 2013-01-01T00:00:00Z20132025-04-26T10:28:00.011Z2019-03-26T23:19:55ZS-EPMC35825592346892610.1371/journal.pone.0057171