{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["8(2)"],"submitter":["Silvent J"],"pubmed_abstract":["Among persisting questions on bone calcification, a major one is the link between protein expression and mineral deposition. A cell culture system is here proposed opening new integrative studies on biomineralization, improving our knowledge on the role played by non-collagenous proteins in bone. This experimental in vitro model consisted in human primary osteoblasts cultured for 60 days at the surface of a 3D collagen scaffold mimicking an osteoid matrix. Various techniques were used to analyze the results at the cellular and molecular level (adhesion and viability tests, histology and electron microscopy, RT- and qPCR) and to characterize the mineral phase (histological staining, EDX, ATG, SAED and RMN). On long term cultures human bone cells seeded on the osteoid-like matrix displayed a"],"journal":["PloS one"],"pagination":["e57344"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3583827"],"repository":["biostudies-literature"],"pubmed_title":["Collagen osteoid-like model allows kinetic gene expression studies of non-collagenous proteins in relation with mineral development to understand bone biomineralization."],"pmcid":["PMC3583827"],"pubmed_authors":["Sire JY","Nassif N","Guille MM","Azais T","Helary C","Silvent J"],"additional_accession":[]},"is_claimable":false,"name":"Collagen osteoid-like model allows kinetic gene expression studies of non-collagenous proteins in relation with mineral development to understand bone biomineralization.","description":"Among persisting questions on bone calcification, a major one is the link between protein expression and mineral deposition. A cell culture system is here proposed opening new integrative studies on biomineralization, improving our knowledge on the role played by non-collagenous proteins in bone. This experimental in vitro model consisted in human primary osteoblasts cultured for 60 days at the surface of a 3D collagen scaffold mimicking an osteoid matrix. Various techniques were used to analyze the results at the cellular and molecular level (adhesion and viability tests, histology and electron microscopy, RT- and qPCR) and to characterize the mineral phase (histological staining, EDX, ATG, SAED and RMN). On long term cultures human bone cells seeded on the osteoid-like matrix displayed a","dates":{"release":"2013-01-01T00:00:00Z","publication":"2013","modification":"2025-04-26T10:28:10.82Z","creation":"2019-03-26T23:19:56Z"},"accession":"S-EPMC3583827","cross_references":{"pubmed":["23460841"],"doi":["10.1371/journal.pone.0057344"]}}