{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Tholen S"],"funding":["European Research Council"],"pagination":["611-25"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3591655"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(3)"],"pubmed_abstract":["Numerous studies highlight the fact that concerted proteolysis is essential for skin morphology and function. The cysteine protease cathepsin L (Ctsl) has been implicated in epidermal proliferation and desquamation, as well as in hair cycle regulation. In stark contrast, mice deficient in cathepsin B (Ctsb) do not display an overt skin phenotype. To understand the systematic consequences of deleting Ctsb or Ctsl, we determined the protein abundances of >1300 proteins and proteolytic cleavage events in skin samples of wild-type, Ctsb(-/-), and Ctsl(-/-) mice via mass-spectrometry-based proteomics. Both protease deficiencies revealed distinct quantitative changes in proteome composition. Ctsl(-/-) skin revealed increased levels of the cysteine protease inhibitors cystatin B and cystatin M/E,"],"journal":["Molecular & cellular proteomics : MCP"],"pubmed_title":["Deletion of cysteine cathepsins B or L yields differential impacts on murine skin proteome and degradome."],"pmcid":["PMC3591655"],"funding_grant_id":["282111"],"pubmed_authors":["Kizhakkedathu JN","Reinheckel T","Boerries M","Busch H","Biniossek ML","Tholen S","Gansz M","Gomez-Auli A","Noel A","Schilling O","Bengsch F"],"additional_accession":[]},"is_claimable":false,"name":"Deletion of cysteine cathepsins B or L yields differential impacts on murine skin proteome and degradome.","description":"Numerous studies highlight the fact that concerted proteolysis is essential for skin morphology and function. The cysteine protease cathepsin L (Ctsl) has been implicated in epidermal proliferation and desquamation, as well as in hair cycle regulation. In stark contrast, mice deficient in cathepsin B (Ctsb) do not display an overt skin phenotype. To understand the systematic consequences of deleting Ctsb or Ctsl, we determined the protein abundances of >1300 proteins and proteolytic cleavage events in skin samples of wild-type, Ctsb(-/-), and Ctsl(-/-) mice via mass-spectrometry-based proteomics. Both protease deficiencies revealed distinct quantitative changes in proteome composition. Ctsl(-/-) skin revealed increased levels of the cysteine protease inhibitors cystatin B and cystatin M/E,","dates":{"release":"2013-01-01T00:00:00Z","publication":"2013 Mar","modification":"2026-05-01T05:08:23.078Z","creation":"2026-04-07T16:40:55.655Z"},"accession":"S-EPMC3591655","cross_references":{"pubmed":["23233448"],"doi":["10.1074/mcp.m112.017962","10.1074/mcp.M112.017962"]}}