{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["110(10)"],"submitter":["Balderhaar HJ"],"pubmed_abstract":["Membrane fusion along the endocytic pathway occurs in a sequence of tethering, docking, and fusion. At endosomes and vacuoles, the CORVET (class C core vacuole/endosome tethering) and HOPS (homotypic fusion and vacuole protein sorting) tethering complexes require their organelle-specific Rabs for localization and function. Until now, despite the absence of experimental evidence, it has been assumed that CORVET is a membrane-tethering factor. To test this theory and understand the mechanistic analogies with the HOPS complex, we set up an in vitro system, and establish CORVET as a bona-fide tether for Vps21-positive endosome/vacuole membranes. Purified CORVET binds to SNAREs and Rab5/Vps21-GTP. We then demonstrate that purified CORVET can specifically tether Vps21-positive membranes. Tetheri"],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pagination":["3823-8"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3593874"],"repository":["biostudies-literature"],"pubmed_title":["The CORVET complex promotes tethering and fusion of Rab5/Vps21-positive membranes."],"pmcid":["PMC3593874"],"pubmed_authors":["Balderhaar HJ","Yavavli E","Brocker C","Lurick A","Lachmann J","Ungermann C"],"additional_accession":[]},"is_claimable":false,"name":"The CORVET complex promotes tethering and fusion of Rab5/Vps21-positive membranes.","description":"Membrane fusion along the endocytic pathway occurs in a sequence of tethering, docking, and fusion. At endosomes and vacuoles, the CORVET (class C core vacuole/endosome tethering) and HOPS (homotypic fusion and vacuole protein sorting) tethering complexes require their organelle-specific Rabs for localization and function. Until now, despite the absence of experimental evidence, it has been assumed that CORVET is a membrane-tethering factor. To test this theory and understand the mechanistic analogies with the HOPS complex, we set up an in vitro system, and establish CORVET as a bona-fide tether for Vps21-positive endosome/vacuole membranes. Purified CORVET binds to SNAREs and Rab5/Vps21-GTP. We then demonstrate that purified CORVET can specifically tether Vps21-positive membranes. Tetheri","dates":{"release":"2013-01-01T00:00:00Z","publication":"2013 Mar","modification":"2025-04-19T09:06:05.192Z","creation":"2019-03-27T01:05:53Z"},"accession":"S-EPMC3593874","cross_references":{"pubmed":["23417307"],"doi":["10.1073/pnas.1221785110"]}}