{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["South PF"],"funding":["NIGMS NIH HHS"],"pagination":["E1016-25"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3600464"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["110(11)"],"pubmed_abstract":["Set1 is a conserved histone H3 lysine 4 (H3K4) methyltransferase that exists as a multisubunit complex. Although H3K4 methylation is located on many actively transcribed genes, few studies have established a direct connection showing that loss of Set1 and H3K4 methylation results in a phenotype caused by disruption of gene expression. In this study, we determined that cells lacking Set1 or Set1 complex members that disrupt H3K4 methylation have a growth defect when grown in the presence of the antifungal drug Brefeldin A (BFA), indicating that H3K4 methylation is needed for BFA resistance. To determine the role of Set1 in BFA resistance, we discovered that Set1 is important for the expression of genes in the ergosterol biosynthetic pathway, including the rate-limiting enzyme HMG-CoA reduct"],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pubmed_title":["H3K4 methyltransferase Set1 is involved in maintenance of ergosterol homeostasis and resistance to Brefeldin A."],"pmcid":["PMC3600464"],"funding_grant_id":["R01 GM074183","GM74183"],"pubmed_authors":["South PF","Briggs SD","Serratore ND","Harmeyer KM"],"additional_accession":[]},"is_claimable":false,"name":"H3K4 methyltransferase Set1 is involved in maintenance of ergosterol homeostasis and resistance to Brefeldin A.","description":"Set1 is a conserved histone H3 lysine 4 (H3K4) methyltransferase that exists as a multisubunit complex. Although H3K4 methylation is located on many actively transcribed genes, few studies have established a direct connection showing that loss of Set1 and H3K4 methylation results in a phenotype caused by disruption of gene expression. In this study, we determined that cells lacking Set1 or Set1 complex members that disrupt H3K4 methylation have a growth defect when grown in the presence of the antifungal drug Brefeldin A (BFA), indicating that H3K4 methylation is needed for BFA resistance. To determine the role of Set1 in BFA resistance, we discovered that Set1 is important for the expression of genes in the ergosterol biosynthetic pathway, including the rate-limiting enzyme HMG-CoA reduct","dates":{"release":"2013-01-01T00:00:00Z","publication":"2013 Mar","modification":"2025-04-04T11:33:12.967Z","creation":"2019-03-27T01:06:14Z"},"accession":"S-EPMC3600464","cross_references":{"pubmed":["23382196"],"doi":["10.1073/pnas.1215768110"]}}