biostudies-literatureMalfait AMNIAMS NIH HHS1042-8https://www.ebi.ac.uk/biostudies/studies/S-EPMC3603144biostudies-literatureUnknown71(6)<h4>Objectives</h4>The aim of this study was to assess if genetic variation in the PACE4 (paired amino acid converting enzyme 4) gene Pcsk6 influences the risk for symptomatic knee osteoarthritis (OA).<h4>Methods</h4>Ten PCSK6 single nucleotide polymorphisms were tested for association in a discovery cohort of radiographic knee OA (n=156 asymptomatic and 600 symptomatic cases). Meta-analysis of the minor allele at rs900414 was performed in three additional independent cohorts (total n=674 asymptomatic and 2068 symptomatic). Pcsk6 knockout mice and wild-type C57BL/6 mice were compared in a battery of algesiometric assays, including hypersensitivity in response to intraplantar substance P, pain behaviours in response to intrathecal substance P and pain behaviour in the abdominal constrictionAnnals of the rheumatic diseasesA role for PACE4 in osteoarthritis pain: evidence from human genetic association and null mutant phenotype.PMC3603144R01AR060364-01R01 AR060364Valdes AMDas RSeymour ABWood LSSorge RELe Graverand-Gastineau MPLeaverton PEHart DJMogil JSMalfait AMDoherty SGao FSotocinal SGSchorscher-Petcu ATortorella MDZhang WDoherty MVaughn FLMuir KRSpector TDArden NKMaciewicz RAfalseA role for PACE4 in osteoarthritis pain: evidence from human genetic association and null mutant phenotype.<h4>Objectives</h4>The aim of this study was to assess if genetic variation in the PACE4 (paired amino acid converting enzyme 4) gene Pcsk6 influences the risk for symptomatic knee osteoarthritis (OA).<h4>Methods</h4>Ten PCSK6 single nucleotide polymorphisms were tested for association in a discovery cohort of radiographic knee OA (n=156 asymptomatic and 600 symptomatic cases). Meta-analysis of the minor allele at rs900414 was performed in three additional independent cohorts (total n=674 asymptomatic and 2068 symptomatic). Pcsk6 knockout mice and wild-type C57BL/6 mice were compared in a battery of algesiometric assays, including hypersensitivity in response to intraplantar substance P, pain behaviours in response to intrathecal substance P and pain behaviour in the abdominal constriction2012-01-01T00:00:00Z2012 Jun2026-05-02T18:26:55.538Z2026-04-07T18:19:43.025ZS-EPMC36031442244082710.1136/annrheumdis-2011-200300