{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Caraballo JC"],"funding":["NCRR NIH HHS","NIEHS NIH HHS","NHLBI NIH HHS"],"pagination":["306-13"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3604088"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["48(3)"],"pubmed_abstract":["Recently, we reported that diesel exhaust particles (DEPs) disrupt tight junctions (TJs) in alveolar epithelial cells (AECs) via an increase in reactive oxygen species (ROS). In this study, we investigated the role of protein kinase C (PKC)-ζ activation in DEP-induced lung injury. C57/bl6 mice were instilled intratracheally with 50 μl of saline containing 100 μg of DEPs or titanium dioxide (TiO2). Twenty-four hours later, bronchoalveolar lavage was performed to assess neutrophil counts and protein concentrations. In addition, in vitro experiments were performed in primary rat and human AECs exposed to DEPs (50 μg/cm(2)) for 3 hours. Transepithelial electrical conductance was measured, and TJ protein association was analyzed by immunoprecipitation. To determine whether the overexpression of"],"journal":["American journal of respiratory cell and molecular biology"],"pubmed_title":["Protein kinase C-ζ mediates lung injury induced by diesel exhaust particles."],"pmcid":["PMC3604088"],"funding_grant_id":["UL1 RR024979","KO1HL080966","UL1RR024979","P30 ES005605","K01 HL080966"],"pubmed_authors":["Thorne PS","Borcherding J","Comellas AP","Caraballo JC"],"additional_accession":[]},"is_claimable":false,"name":"Protein kinase C-ζ mediates lung injury induced by diesel exhaust particles.","description":"Recently, we reported that diesel exhaust particles (DEPs) disrupt tight junctions (TJs) in alveolar epithelial cells (AECs) via an increase in reactive oxygen species (ROS). In this study, we investigated the role of protein kinase C (PKC)-ζ activation in DEP-induced lung injury. C57/bl6 mice were instilled intratracheally with 50 μl of saline containing 100 μg of DEPs or titanium dioxide (TiO2). Twenty-four hours later, bronchoalveolar lavage was performed to assess neutrophil counts and protein concentrations. In addition, in vitro experiments were performed in primary rat and human AECs exposed to DEPs (50 μg/cm(2)) for 3 hours. Transepithelial electrical conductance was measured, and TJ protein association was analyzed by immunoprecipitation. To determine whether the overexpression of","dates":{"release":"2013-01-01T00:00:00Z","publication":"2013 Mar","modification":"2025-04-22T09:06:07.15Z","creation":"2019-03-27T01:06:22Z"},"accession":"S-EPMC3604088","cross_references":{"pubmed":["23221045"],"doi":["10.1165/rcmb.2012-0056OC","10.1165/rcmb.2012-0056oc"]}}