{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Domanski M"],"funding":["NCRR NIH HHS","NIGMS NIH HHS"],"pagination":["1-6"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3605737"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["0(0)"],"pubmed_abstract":["An efficient and reliable procedure for the capture of affinity-tagged proteins and associated complexes from human cell lines is reported. Through multiple optimizations, high yield and low background affinity-purifications are achieved from modest quantities of human cells expressing endogenous-level tagged proteins. Isolations of triple-FLAG and GFP-tagged fusion proteins involved in RNA metabolism are presented."],"journal":["BioTechniques"],"pubmed_title":["Improved methodology for the affinity isolation of human protein complexes expressed at near endogenous levels."],"pmcid":["PMC3605737"],"funding_grant_id":["U54 RR022220","U54 GM103511"],"pubmed_authors":["Jensen TH","LaCava J","Chait BT","Jiang H","Molloy K","Domanski M","Rout MP"],"additional_accession":[]},"is_claimable":false,"name":"Improved methodology for the affinity isolation of human protein complexes expressed at near endogenous levels.","description":"An efficient and reliable procedure for the capture of affinity-tagged proteins and associated complexes from human cell lines is reported. Through multiple optimizations, high yield and low background affinity-purifications are achieved from modest quantities of human cells expressing endogenous-level tagged proteins. Isolations of triple-FLAG and GFP-tagged fusion proteins involved in RNA metabolism are presented.","dates":{"release":"2012-01-01T00:00:00Z","publication":"2012 May","modification":"2025-04-25T22:00:02.245Z","creation":"2019-03-27T01:06:29Z"},"accession":"S-EPMC3605737","cross_references":{"pubmed":["22668517"],"doi":["10.2144/000113864"]}}