biostudies-literatureDomanski MNCRR NIH HHSNIGMS NIH HHS1-6https://www.ebi.ac.uk/biostudies/studies/S-EPMC3605737biostudies-literatureUnknown0(0)An efficient and reliable procedure for the capture of affinity-tagged proteins and associated complexes from human cell lines is reported. Through multiple optimizations, high yield and low background affinity-purifications are achieved from modest quantities of human cells expressing endogenous-level tagged proteins. Isolations of triple-FLAG and GFP-tagged fusion proteins involved in RNA metabolism are presented.BioTechniquesImproved methodology for the affinity isolation of human protein complexes expressed at near endogenous levels.PMC3605737U54 RR022220U54 GM103511Jensen THLaCava JChait BTJiang HMolloy KDomanski MRout MPfalseImproved methodology for the affinity isolation of human protein complexes expressed at near endogenous levels.An efficient and reliable procedure for the capture of affinity-tagged proteins and associated complexes from human cell lines is reported. Through multiple optimizations, high yield and low background affinity-purifications are achieved from modest quantities of human cells expressing endogenous-level tagged proteins. Isolations of triple-FLAG and GFP-tagged fusion proteins involved in RNA metabolism are presented.2012-01-01T00:00:00Z2012 May2025-04-25T22:00:02.245Z2019-03-27T01:06:29ZS-EPMC36057372266851710.2144/000113864