{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["3"],"submitter":["Mabalirajan U"],"pubmed_abstract":["The mechanisms underlying asthmatic airway epithelial injury are not clear. 12/15-lipoxygenase (an ortholog of human 15-LOX-1), which is induced by IL-13, is associated with mitochondrial degradation in reticulocytes at physiological conditions. In this study, we showed that 12/15-LOX expressed in nonepithelial cells caused epithelial injury in asthma pathogenesis. While 12/15-LOX overexpression or IL-13 administration to naïve mice showed airway epithelial injury, 12/15-LOX knockout/knockdown in allergic mice reduced airway epithelial injury. The constitutive expression of 15-LOX-1 in bronchial epithelia of normal human lungs further indicated that epithelial 15-LOX-1 may not cause epithelial injury. 12/15-LOX expression is increased in various inflammatory cells in allergic mice. Though non-epithelial cells such as macrophages or fibroblasts released 12/15-LOX metabolites upon IL-13 induction, bronchial epithelia didn't release. Further 12-S-HETE, arachidonic acid metabolite of 12/15-LOX leads to epithelial injury. These findings suggested 12/15-LOX expressed in non-epithelial cells such as macrophages and fibroblasts leads to bronchial epithelial injury."],"journal":["Scientific reports"],"pagination":["1540"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3607899"],"repository":["biostudies-literature"],"pubmed_title":["12/15-lipoxygenase expressed in non-epithelial cells causes airway epithelial injury in asthma."],"pmcid":["PMC3607899"],"pubmed_authors":["Ghosh B","Leishangthem GD","Dinda AK","Ahmad T","Biswal S","Rehman R","Singh S","Mabalirajan U","Agrawal A","Kumar S"],"additional_accession":[]},"is_claimable":false,"name":"12/15-lipoxygenase expressed in non-epithelial cells causes airway epithelial injury in asthma.","description":"The mechanisms underlying asthmatic airway epithelial injury are not clear. 12/15-lipoxygenase (an ortholog of human 15-LOX-1), which is induced by IL-13, is associated with mitochondrial degradation in reticulocytes at physiological conditions. In this study, we showed that 12/15-LOX expressed in nonepithelial cells caused epithelial injury in asthma pathogenesis. While 12/15-LOX overexpression or IL-13 administration to naïve mice showed airway epithelial injury, 12/15-LOX knockout/knockdown in allergic mice reduced airway epithelial injury. The constitutive expression of 15-LOX-1 in bronchial epithelia of normal human lungs further indicated that epithelial 15-LOX-1 may not cause epithelial injury. 12/15-LOX expression is increased in various inflammatory cells in allergic mice. Though non-epithelial cells such as macrophages or fibroblasts released 12/15-LOX metabolites upon IL-13 induction, bronchial epithelia didn't release. Further 12-S-HETE, arachidonic acid metabolite of 12/15-LOX leads to epithelial injury. These findings suggested 12/15-LOX expressed in non-epithelial cells such as macrophages and fibroblasts leads to bronchial epithelial injury.","dates":{"release":"2013-01-01T00:00:00Z","publication":"2013","modification":"2025-04-18T14:15:58.741Z","creation":"2019-03-27T01:06:37Z"},"accession":"S-EPMC3607899","cross_references":{"pubmed":["23528921"],"doi":["10.1038/srep01540"]}}