{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Trinh HV"],"funding":["University of Zurich Forschungskredit"],"pagination":["581862"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3608280"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["2013"],"pubmed_abstract":["Both isobaric tags for relative and absolute quantitation (iTRAQ) and label-free methods are widely used for quantitative proteomics. Here, we provide a detailed evaluation of these proteomics approaches based on large datasets from biological samples. iTRAQ-label-based and label-free quantitations were compared using protein lysate samples from noninfected human lung epithelial A549 cells and from cells infected for 24 h with human adenovirus type 3 or type 5. Either iTRAQ-label-based or label-free methods were used, and the resulting samples were analyzed by liquid chromatography (LC) and tandem mass spectrometry (MS/MS). To reduce a possible bias from quantitation software, we applied several software packages for each procedure. ProteinPilot and Scaffold Q+ software were used for iTRAQ"],"journal":["International journal of proteomics"],"pubmed_title":["iTRAQ-Based and Label-Free Proteomics Approaches for Studies of Human Adenovirus Infections."],"pmcid":["PMC3608280"],"funding_grant_id":["31003A_141222","57113401"],"pubmed_authors":["Hemmi S","Schlapbach R","Greber UF","Trinh HV","Roschitzki B","Grossmann J","Gehrig P"],"additional_accession":[]},"is_claimable":false,"name":"iTRAQ-Based and Label-Free Proteomics Approaches for Studies of Human Adenovirus Infections.","description":"Both isobaric tags for relative and absolute quantitation (iTRAQ) and label-free methods are widely used for quantitative proteomics. Here, we provide a detailed evaluation of these proteomics approaches based on large datasets from biological samples. iTRAQ-label-based and label-free quantitations were compared using protein lysate samples from noninfected human lung epithelial A549 cells and from cells infected for 24 h with human adenovirus type 3 or type 5. Either iTRAQ-label-based or label-free methods were used, and the resulting samples were analyzed by liquid chromatography (LC) and tandem mass spectrometry (MS/MS). To reduce a possible bias from quantitation software, we applied several software packages for each procedure. ProteinPilot and Scaffold Q+ software were used for iTRAQ","dates":{"release":"2013-01-01T00:00:00Z","publication":"2013","modification":"2026-04-30T06:18:00.914Z","creation":"2019-03-27T01:06:37Z"},"accession":"S-EPMC3608280","cross_references":{"pubmed":["23555056"],"doi":["10.1155/2013/581862"]}}