biostudies-literatureTrinh HVUniversity of Zurich Forschungskredit581862https://www.ebi.ac.uk/biostudies/studies/S-EPMC3608280biostudies-literatureUnknown2013Both isobaric tags for relative and absolute quantitation (iTRAQ) and label-free methods are widely used for quantitative proteomics. Here, we provide a detailed evaluation of these proteomics approaches based on large datasets from biological samples. iTRAQ-label-based and label-free quantitations were compared using protein lysate samples from noninfected human lung epithelial A549 cells and from cells infected for 24 h with human adenovirus type 3 or type 5. Either iTRAQ-label-based or label-free methods were used, and the resulting samples were analyzed by liquid chromatography (LC) and tandem mass spectrometry (MS/MS). To reduce a possible bias from quantitation software, we applied several software packages for each procedure. ProteinPilot and Scaffold Q+ software were used for iTRAQInternational journal of proteomicsiTRAQ-Based and Label-Free Proteomics Approaches for Studies of Human Adenovirus Infections.PMC360828031003A_14122257113401Hemmi SSchlapbach RGreber UFTrinh HVRoschitzki BGrossmann JGehrig PfalseiTRAQ-Based and Label-Free Proteomics Approaches for Studies of Human Adenovirus Infections.Both isobaric tags for relative and absolute quantitation (iTRAQ) and label-free methods are widely used for quantitative proteomics. Here, we provide a detailed evaluation of these proteomics approaches based on large datasets from biological samples. iTRAQ-label-based and label-free quantitations were compared using protein lysate samples from noninfected human lung epithelial A549 cells and from cells infected for 24 h with human adenovirus type 3 or type 5. Either iTRAQ-label-based or label-free methods were used, and the resulting samples were analyzed by liquid chromatography (LC) and tandem mass spectrometry (MS/MS). To reduce a possible bias from quantitation software, we applied several software packages for each procedure. ProteinPilot and Scaffold Q+ software were used for iTRAQ2013-01-01T00:00:00Z20132026-04-30T06:18:00.914Z2019-03-27T01:06:37ZS-EPMC36082802355505610.1155/2013/581862