biostudies-literatureDillman AAIntramural NIH HHSNIA NIH HHS499-506https://www.ebi.ac.uk/biostudies/studies/S-EPMC3609882biostudies-literatureUnknown16(4)The complexity of the adult brain is a result of both developmental processes and experience-dependent circuit formation. One way to look at the differences between embryonic and adult brain is to examine gene expression. Previous studies have used microarrays to address this in a global manner. However, the transcriptome is more complex than gene expression levels alone, as alternative splicing and RNA editing generate a diverse set of mature transcripts. Here we report a high-resolution transcriptome data set of mouse cerebral cortex at embryonic and adult stages using RNA sequencing (RNA-Seq). We found many differences in gene expression, splicing and RNA editing between embryonic and adult cerebral cortex. Each data set was validated technically and biologically, and in each case we found our RNA-Seq observations to have predictive validity. We provide this data set and analysis as a resource for understanding gene expression in the embryonic and adult cerebral cortex.Nature neurosciencemRNA expression, splicing and editing in the embryonic and adult mouse cerebral cortex.PMC3609882K01 AG000947AG000947ZIA AG000947-05Gibbs JRRudenko INDillman AACookson MRMcCoy MKHauser DNNalls MAGalter DfalsemRNA expression, splicing and editing in the embryonic and adult mouse cerebral cortex.The complexity of the adult brain is a result of both developmental processes and experience-dependent circuit formation. One way to look at the differences between embryonic and adult brain is to examine gene expression. Previous studies have used microarrays to address this in a global manner. However, the transcriptome is more complex than gene expression levels alone, as alternative splicing and RNA editing generate a diverse set of mature transcripts. Here we report a high-resolution transcriptome data set of mouse cerebral cortex at embryonic and adult stages using RNA sequencing (RNA-Seq). We found many differences in gene expression, splicing and RNA editing between embryonic and adult cerebral cortex. Each data set was validated technically and biologically, and in each case we found our RNA-Seq observations to have predictive validity. We provide this data set and analysis as a resource for understanding gene expression in the embryonic and adult cerebral cortex.2013-01-01T00:00:00Z2013 Apr2021-02-19T18:09:26Z2019-03-27T01:06:40ZS-EPMC36098822341645210.1038/nn.3332