biostudies-literatureUnknown3Liu GLong-term strain improvements through repeated mutagenesis and screening have generated a hyper-producer of cellulases and hemicellulases from Penicillium decumbens 114 which was isolated 30 years ago. Here, the genome of the hyper-producer P. decumbens JU-A10-T was sequenced and compared with that of the wild-type strain 114-2. Further, the transcriptomes and secretomes were compared between the strains. Selective hyper-production of cellulases and hemicellulases but not all the secreted proteins was observed in the mutant, making it a more specific producer of lignocellulolytic enzymes. Functional analysis identified that changes in several transcriptional regulatory elements played crucial roles in the cellulase hyper-producing characteristics of the mutant. Additionally, the mutant shoScientific reports1569https://www.ebi.ac.uk/biostudies/studies/S-EPMC3610096biostudies-literatureLong-term strain improvements accumulate mutations in regulatory elements responsible for hyper-production of cellulolytic enzymes.PMC3610096Ma LZhang JWang CQin YWei XLiu RZhang LZou GWang LLiu WZheng KZheng HXun LWang SWang TQu YLi JChen MChen LBao XZhong YXu HYan XZhou ZFang XLi ZLiu GZhao GPfalseLong-term strain improvements accumulate mutations in regulatory elements responsible for hyper-production of cellulolytic enzymes.Long-term strain improvements through repeated mutagenesis and screening have generated a hyper-producer of cellulases and hemicellulases from Penicillium decumbens 114 which was isolated 30 years ago. Here, the genome of the hyper-producer P. decumbens JU-A10-T was sequenced and compared with that of the wild-type strain 114-2. Further, the transcriptomes and secretomes were compared between the strains. Selective hyper-production of cellulases and hemicellulases but not all the secreted proteins was observed in the mutant, making it a more specific producer of lignocellulolytic enzymes. Functional analysis identified that changes in several transcriptional regulatory elements played crucial roles in the cellulase hyper-producing characteristics of the mutant. Additionally, the mutant sho2013-01-01T00:00:00Z20132026-04-29T19:22:02.505Z2026-04-07T15:26:16.941ZS-EPMC36100962353583810.1038/srep01569