{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Li XA"],"funding":["NCI NIH HHS"],"pagination":["3446-53"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC361074"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["11(7)"],"pubmed_abstract":["GRP78, a 78-kDa protein localized in the endoplasmic reticulum (ER), has been implicated in protein processing and stress protection. Its promoter contains a 36-bp region which is conserved among GRP genes across species and has the ability to compete for trans-acting factors mediating GRP gene expression. Integration of about 800 tandem copies of this sequence into the genome of a Chinese hamster ovary cell line (DG44) results in transfectants with the following phenotypes: (i) the induction level of GRP78 by the calcium ionophore A23187 and tunicamycin is reduced 4- and 2-fold, respectively, (ii) the induction levels of two other ER luminal protein genes, GRP94 and ERp72, are simultaneously down-regulated, (iii) the growth rate of these cells is half that of transfectants without the amp"],"journal":["Molecular and cellular biology"],"pubmed_title":["Competitive inhibition of a set of endoplasmic reticulum protein genes (GRP78, GRP94, and ERp72) retards cell growth and lowers viability after ionophore treatment."],"pmcid":["PMC361074"],"funding_grant_id":["R37 CA27607"],"pubmed_authors":["Li XA","Lee AS"],"additional_accession":[]},"is_claimable":false,"name":"Competitive inhibition of a set of endoplasmic reticulum protein genes (GRP78, GRP94, and ERp72) retards cell growth and lowers viability after ionophore treatment.","description":"GRP78, a 78-kDa protein localized in the endoplasmic reticulum (ER), has been implicated in protein processing and stress protection. Its promoter contains a 36-bp region which is conserved among GRP genes across species and has the ability to compete for trans-acting factors mediating GRP gene expression. Integration of about 800 tandem copies of this sequence into the genome of a Chinese hamster ovary cell line (DG44) results in transfectants with the following phenotypes: (i) the induction level of GRP78 by the calcium ionophore A23187 and tunicamycin is reduced 4- and 2-fold, respectively, (ii) the induction levels of two other ER luminal protein genes, GRP94 and ERp72, are simultaneously down-regulated, (iii) the growth rate of these cells is half that of transfectants without the amp","dates":{"release":"1991-01-01T00:00:00Z","publication":"1991 Jul","modification":"2025-04-04T09:47:11.006Z","creation":"2019-03-27T00:50:09Z"},"accession":"S-EPMC361074","cross_references":{"pubmed":["2046663"],"doi":["10.1128/mcb.11.7.3446-3453.1991"]}}