{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Hawkins KE"],"funding":["NINDS NIH HHS"],"pagination":["14"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3620676"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["6"],"pubmed_abstract":["<h4>Background</h4>Matrix metalloproteinases are important factors in the molecular mechanisms leading to neuronal injury in many neurological disorders. Matrix metalloproteinase (MMP)-9 is up-regulated after cerebral ischemia and neuroinflammation and is actively involved in blood-brain barrier disruption. Current methods of measuring MMP-9 activity, such as gelatin-substrate zymography, are unspecific and arduous. Here we developed an immunocapture assay with high efficiency, specificity, and sensitivity for quantifying endogenously active as well as total MMP-9 activity.<h4>Results</h4>A fluorescence resonance energy transfer (FRET) peptide-based immunocapture assay was developed that enables the accurate assessment of total and active forms of MMP-9 in complex biological samples. The F"],"journal":["Molecular brain"],"pubmed_title":["Fluorometric immunocapture assay for the specific measurement of matrix metalloproteinase-9 activity in biological samples: application to brain and plasma from rats with ischemic stroke."],"pmcid":["PMC3620676"],"funding_grant_id":["R01 NS065849"],"pubmed_authors":["Candelario-Jalil E","Rosenberg GA","Hawkins KE","DeMars KM","Yang C"],"additional_accession":[]},"is_claimable":false,"name":"Fluorometric immunocapture assay for the specific measurement of matrix metalloproteinase-9 activity in biological samples: application to brain and plasma from rats with ischemic stroke.","description":"<h4>Background</h4>Matrix metalloproteinases are important factors in the molecular mechanisms leading to neuronal injury in many neurological disorders. Matrix metalloproteinase (MMP)-9 is up-regulated after cerebral ischemia and neuroinflammation and is actively involved in blood-brain barrier disruption. Current methods of measuring MMP-9 activity, such as gelatin-substrate zymography, are unspecific and arduous. Here we developed an immunocapture assay with high efficiency, specificity, and sensitivity for quantifying endogenously active as well as total MMP-9 activity.<h4>Results</h4>A fluorescence resonance energy transfer (FRET) peptide-based immunocapture assay was developed that enables the accurate assessment of total and active forms of MMP-9 in complex biological samples. The F","dates":{"release":"2013-01-01T00:00:00Z","publication":"2013 Mar","modification":"2025-04-26T10:32:24.794Z","creation":"2019-03-27T01:07:10Z"},"accession":"S-EPMC3620676","cross_references":{"pubmed":["23522154"],"doi":["10.1186/1756-6606-6-14"]}}