biostudies-literatureStewart ANIAID NIH HHSNIGMS NIH HHS9https://www.ebi.ac.uk/biostudies/studies/S-EPMC3626704biostudies-literatureUnknown14<h4>Background</h4>Analysis of factors contributing to high affinity antibody-protein interactions provides insight into natural antibody evolution, and guides the design of antibodies with new or enhanced function. We previously studied the interaction between antibody D5 and its target, a designed protein based on HIV-1 gp41 known as 5-Helix, as a model system [Da Silva, G. F.; Harrison, J. S.; Lai, J. R., Biochemistry, 2010, 49, 5464-5472]. Antibody D5 represents an interesting case study because it is derived from the VH1-69 germline segment; this germline segment is characterized by a hydrophobic second heavy chain complementarity determining region (HCDR2) that constitutes the major functional paratope in D5 and several antibodies derived from the same progenitor.<h4>Results</h4>HereBMC biochemistrySide chain requirements for affinity and specificity in D5, an HIV-1 antibody derived from the VH1-69 germline segment.PMC3626704P30-AI51519R01-AI090249T32-GM008572R01 AI090249Regula LKLai JRHarrison JSStewart AfalseSide chain requirements for affinity and specificity in D5, an HIV-1 antibody derived from the VH1-69 germline segment.<h4>Background</h4>Analysis of factors contributing to high affinity antibody-protein interactions provides insight into natural antibody evolution, and guides the design of antibodies with new or enhanced function. We previously studied the interaction between antibody D5 and its target, a designed protein based on HIV-1 gp41 known as 5-Helix, as a model system [Da Silva, G. F.; Harrison, J. S.; Lai, J. R., Biochemistry, 2010, 49, 5464-5472]. Antibody D5 represents an interesting case study because it is derived from the VH1-69 germline segment; this germline segment is characterized by a hydrophobic second heavy chain complementarity determining region (HCDR2) that constitutes the major functional paratope in D5 and several antibodies derived from the same progenitor.<h4>Results</h4>Here2013-01-01T00:00:00Z2013 Apr2025-04-04T11:48:02.065Z2019-03-27T01:07:26ZS-EPMC36267042356619810.1186/1471-2091-14-9