{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kalesnykas G"],"funding":["NEI NIH HHS"],"pagination":["3847-57"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3630905"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["53(7)"],"pubmed_abstract":["<h4>Purpose</h4>To study sequential changes in retinal ganglion cell (RGC) morphology in mice after optic nerve crush and after induction of experimental glaucoma.<h4>Methods</h4>Nerve crush or experimental glaucoma was induced in mice that selectively express yellow fluorescent protein (YFP) in RGCs. Mice were euthanized 1, 4, and 9 days after crush and 1, 3, and 6 weeks after induction of glaucoma by bead injection. All YFP-RGCs were identified in retinal whole mounts. Then confocal images of randomly selected RGCs were quantified for somal fluorescence brightness, soma size, neurite outgrowth, and dendritic complexity (Sholl analysis).<h4>Results</h4>By 9 days after crush, 98% of RGC axons died and YFP-RGCs decreased by 64%. After 6 weeks of glaucoma, 31% of axons died, but there was no"],"journal":["Investigative ophthalmology & visual science"],"pubmed_title":["Retinal ganglion cell morphology after optic nerve crush and experimental glaucoma."],"pmcid":["PMC3630905"],"funding_grant_id":["R56 EY002120","EY-02120","P30 EY001765","R01 EY002120","EY-01765"],"pubmed_authors":["Oglesby EN","Tian J","Pease ME","Quigley HA","Kalesnykas G","Cone FE","Zack DJ","Steinhart MR"],"additional_accession":[]},"is_claimable":false,"name":"Retinal ganglion cell morphology after optic nerve crush and experimental glaucoma.","description":"<h4>Purpose</h4>To study sequential changes in retinal ganglion cell (RGC) morphology in mice after optic nerve crush and after induction of experimental glaucoma.<h4>Methods</h4>Nerve crush or experimental glaucoma was induced in mice that selectively express yellow fluorescent protein (YFP) in RGCs. Mice were euthanized 1, 4, and 9 days after crush and 1, 3, and 6 weeks after induction of glaucoma by bead injection. All YFP-RGCs were identified in retinal whole mounts. Then confocal images of randomly selected RGCs were quantified for somal fluorescence brightness, soma size, neurite outgrowth, and dendritic complexity (Sholl analysis).<h4>Results</h4>By 9 days after crush, 98% of RGC axons died and YFP-RGCs decreased by 64%. After 6 weeks of glaucoma, 31% of axons died, but there was no","dates":{"release":"2012-01-01T00:00:00Z","publication":"2012 Jun","modification":"2025-04-25T22:10:46.201Z","creation":"2019-03-27T01:07:39Z"},"accession":"S-EPMC3630905","cross_references":{"pubmed":["22589442"],"doi":["10.1167/iovs.12-9712"]}}