biostudies-literatureKalesnykas GNEI NIH HHS3847-57https://www.ebi.ac.uk/biostudies/studies/S-EPMC3630905biostudies-literatureUnknown53(7)<h4>Purpose</h4>To study sequential changes in retinal ganglion cell (RGC) morphology in mice after optic nerve crush and after induction of experimental glaucoma.<h4>Methods</h4>Nerve crush or experimental glaucoma was induced in mice that selectively express yellow fluorescent protein (YFP) in RGCs. Mice were euthanized 1, 4, and 9 days after crush and 1, 3, and 6 weeks after induction of glaucoma by bead injection. All YFP-RGCs were identified in retinal whole mounts. Then confocal images of randomly selected RGCs were quantified for somal fluorescence brightness, soma size, neurite outgrowth, and dendritic complexity (Sholl analysis).<h4>Results</h4>By 9 days after crush, 98% of RGC axons died and YFP-RGCs decreased by 64%. After 6 weeks of glaucoma, 31% of axons died, but there was noInvestigative ophthalmology & visual scienceRetinal ganglion cell morphology after optic nerve crush and experimental glaucoma.PMC3630905R56 EY002120EY-02120P30 EY001765R01 EY002120EY-01765Oglesby ENTian JPease MEQuigley HAKalesnykas GCone FEZack DJSteinhart MRfalseRetinal ganglion cell morphology after optic nerve crush and experimental glaucoma.<h4>Purpose</h4>To study sequential changes in retinal ganglion cell (RGC) morphology in mice after optic nerve crush and after induction of experimental glaucoma.<h4>Methods</h4>Nerve crush or experimental glaucoma was induced in mice that selectively express yellow fluorescent protein (YFP) in RGCs. Mice were euthanized 1, 4, and 9 days after crush and 1, 3, and 6 weeks after induction of glaucoma by bead injection. All YFP-RGCs were identified in retinal whole mounts. Then confocal images of randomly selected RGCs were quantified for somal fluorescence brightness, soma size, neurite outgrowth, and dendritic complexity (Sholl analysis).<h4>Results</h4>By 9 days after crush, 98% of RGC axons died and YFP-RGCs decreased by 64%. After 6 weeks of glaucoma, 31% of axons died, but there was no2012-01-01T00:00:00Z2012 Jun2025-04-25T22:10:46.201Z2019-03-27T01:07:39ZS-EPMC36309052258944210.1167/iovs.12-9712