biostudies-literatureKang PJNIGMS NIH HHS1218-26https://www.ebi.ac.uk/biostudies/studies/S-EPMC3635463biostudies-literatureUnknown126(Pt 5)Cells of the budding yeast Saccharomyces cerevisiae select a site for polarized growth in a specific pattern that depends on their cell type. Haploid a and α cells bud in the axial budding pattern, which requires assembly of a landmark that includes the Bud4 protein. To understand how an axial bud site is established, we performed a structure-function analysis of Bud4. Bud4 contains DUF1709 (domain of unknown function), which is similar to a part of the anillin-homology domain, and a putative Pleckstrin homology (PH) domain near to its C terminus. Although its localization depends on septins, a conserved family of GTP-binding proteins, Bud4 is necessary for the stable inheritance of septin rings during cell division. Although some anillins interact directly with septins, we find that neithJournal of cell scienceCoupling of septins to the axial landmark by Bud4 in budding yeast.PMC3635463R01 GM076375R01-GM76375Hood-DeGrenier JKKang PJPark HOfalseCoupling of septins to the axial landmark by Bud4 in budding yeast.Cells of the budding yeast Saccharomyces cerevisiae select a site for polarized growth in a specific pattern that depends on their cell type. Haploid a and α cells bud in the axial budding pattern, which requires assembly of a landmark that includes the Bud4 protein. To understand how an axial bud site is established, we performed a structure-function analysis of Bud4. Bud4 contains DUF1709 (domain of unknown function), which is similar to a part of the anillin-homology domain, and a putative Pleckstrin homology (PH) domain near to its C terminus. Although its localization depends on septins, a conserved family of GTP-binding proteins, Bud4 is necessary for the stable inheritance of septin rings during cell division. Although some anillins interact directly with septins, we find that neith2013-01-01T00:00:00Z2013 Mar2025-04-19T09:07:25.996Z2019-03-27T01:07:50ZS-EPMC36354632334539510.1242/jcs.118521