{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["8(4)"],"submitter":["Cho CP"],"pubmed_abstract":["RNA structures are unwound for decoding. In the process, they can pause the elongating ribosome for regulation. An example is the stimulation of -1 programmed ribosomal frameshifting, leading to 3' direction slippage of the reading-frame during elongation, by specific pseudoknot stimulators downstream of the frameshifting site. By investigating a recently identified regulatory element upstream of the SARS coronavirus (SARS-CoV) -1 frameshifting site, it is shown that a minimal functional element with hairpin forming potential is sufficient to down-regulate-1 frameshifting activity. Mutagenesis to disrupt or restore base pairs in the potential hairpin stem reveals that base-pair formation is required for-1 frameshifting attenuation in vitro and in 293T cells. The attenuation efficiency of a"],"journal":["PloS one"],"pagination":["e62283"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3639245"],"repository":["biostudies-literature"],"pubmed_title":["Regulation of programmed ribosomal frameshifting by co-translational refolding RNA hairpins."],"pmcid":["PMC3639245"],"pubmed_authors":["Cho CP","Chang KY","Lin SC","Hsu HT","Chou MY"],"additional_accession":[]},"is_claimable":false,"name":"Regulation of programmed ribosomal frameshifting by co-translational refolding RNA hairpins.","description":"RNA structures are unwound for decoding. In the process, they can pause the elongating ribosome for regulation. An example is the stimulation of -1 programmed ribosomal frameshifting, leading to 3' direction slippage of the reading-frame during elongation, by specific pseudoknot stimulators downstream of the frameshifting site. By investigating a recently identified regulatory element upstream of the SARS coronavirus (SARS-CoV) -1 frameshifting site, it is shown that a minimal functional element with hairpin forming potential is sufficient to down-regulate-1 frameshifting activity. Mutagenesis to disrupt or restore base pairs in the potential hairpin stem reveals that base-pair formation is required for-1 frameshifting attenuation in vitro and in 293T cells. The attenuation efficiency of a","dates":{"release":"2013-01-01T00:00:00Z","publication":"2013","modification":"2025-04-21T23:40:27.399Z","creation":"2019-03-26T23:14:16Z"},"accession":"S-EPMC3639245","cross_references":{"pubmed":["23638024"],"doi":["10.1371/journal.pone.0062283"]}}