{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Li Q"],"funding":["NHLBI NIH HHS"],"pagination":["849-64"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3640450"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["106(5)"],"pubmed_abstract":["A model of intracoronary stem cell delivery that enables transgenesis/gene targeting would be a powerful tool but is still lacking. To address this gap, we compared intracoronary and intramyocardial delivery of lin(-)/c-kit(+)/GFP(+) cardiac stem cells (CSCs) in a murine model of reperfused myocardial infarction (MI). Lin(-)/c-kit(+)/GFP(+) CSCs were successfully expanded from GFP transgenic hearts and cultured with no detectable phenotypic change for up to ten passages. Intracoronary delivery of CSCs 2 days post-MI resulted in significant alleviation of adverse LV remodeling and dysfunction, which was at least equivalent, if not superior, to that achieved with intramyocardial delivery. Compared with intramyocardial injection, intracoronary infusion was associated with a more homogeneous d"],"journal":["Basic research in cardiology"],"pubmed_title":["Intracoronary administration of cardiac stem cells in mice: a new, improved technique for cell therapy in murine models."],"pmcid":["PMC3640450"],"funding_grant_id":["P01HL78825","R01 HL55757","HL-76794","HL-70897","P01 HL078825","R01 HL055757","R01 HL070897","R01 HL076794"],"pubmed_authors":["Chen N","Ou Q","Yuan F","Guo Y","Wang T","Luo L","Bolli R","O'Brien E","Li Q","Wu WJ","Zhu X","Hunt GN"],"additional_accession":[]},"is_claimable":false,"name":"Intracoronary administration of cardiac stem cells in mice: a new, improved technique for cell therapy in murine models.","description":"A model of intracoronary stem cell delivery that enables transgenesis/gene targeting would be a powerful tool but is still lacking. To address this gap, we compared intracoronary and intramyocardial delivery of lin(-)/c-kit(+)/GFP(+) cardiac stem cells (CSCs) in a murine model of reperfused myocardial infarction (MI). Lin(-)/c-kit(+)/GFP(+) CSCs were successfully expanded from GFP transgenic hearts and cultured with no detectable phenotypic change for up to ten passages. Intracoronary delivery of CSCs 2 days post-MI resulted in significant alleviation of adverse LV remodeling and dysfunction, which was at least equivalent, if not superior, to that achieved with intramyocardial delivery. Compared with intramyocardial injection, intracoronary infusion was associated with a more homogeneous d","dates":{"release":"2011-01-01T00:00:00Z","publication":"2011 Sep","modification":"2025-04-22T01:49:25.082Z","creation":"2019-03-27T01:08:06Z"},"accession":"S-EPMC3640450","cross_references":{"pubmed":["21516491"],"doi":["10.1007/s00395-011-0180-1"]}}